Literature DB >> 7014645

Gas-liquid chromatography in routine processing of blood cultures for detecting anaerobic bacteraemia.

M Reig, D Molina, E Loza, M A Ledesma, M A Meseguer.   

Abstract

Gas-liquid chromatography was performed on 233 positive blood cultures and findings were compared with culture results. Obligate anaerobic bacteria were recovered from 78 out of 79 blood cultures containing butyric or iso-valeric acids, or both; from 28 out of 69 blood cultures containing succinic acid; and from only one out of 41 blood cultures containing succinic but not butyric or iso-valeric acid. Good correlations (88%) were found for the recovery of anaerobic bacteria and the detection of butyric and/or iso-valeric acids. Detecting volatile fatty acids by gas-liquid chromatography performed on blood cultures at the first signs of growth can therefore provide an early and reliable indication of the presence of anaerobic bacteria.

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Year:  1981        PMID: 7014645      PMCID: PMC1146451          DOI: 10.1136/jcp.34.2.189

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN: 0021-9746            Impact factor:   3.411


  10 in total

1.  Modification of the Minitek Miniaturized Differentiation System for characterization of anaerobic bacteria.

Authors:  D Stargel; F S Thompson; S E Phillips; G L Lombard; V R Dowell
Journal:  J Clin Microbiol       Date:  1976-03       Impact factor: 5.948

2.  Rapid diagnosis of anaerobic infections by gas-liquid chromatography of clinical material.

Authors:  K D Phillips; P V Tearle; A T Willis
Journal:  J Clin Pathol       Date:  1976-05       Impact factor: 3.411

3.  Evaluation of routine subcultures of macroscopically negative blood cultures for detection of anaerobes.

Authors:  P R Murray; J E Sondag
Journal:  J Clin Microbiol       Date:  1978-10       Impact factor: 5.948

4.  Evaluation of a routine anaerobic subculture of blood cultures for detection of anaerobic bacteremia.

Authors:  J W Paisley; J E Rosenblatt; M Hall; J A Washington
Journal:  J Clin Microbiol       Date:  1978-12       Impact factor: 5.948

5.  [Gas liquid chromatography in the study of blood cultures].

Authors:  M C Ponte; E Parrilla; F Soriano; J Vega; J M Ales
Journal:  Rev Clin Esp       Date:  1978-11-30       Impact factor: 1.556

6.  Presumptive diagnosis of anaerobic bacteremia by gas-liquid chromatography of blood cultures.

Authors:  J Wüst
Journal:  J Clin Microbiol       Date:  1977-12       Impact factor: 5.948

7.  Rapid diagnosis of anaerobic infections by direct gas-liquid chromatography of clinical speciments.

Authors:  S L Gorbach; J W Mayhew; J G Bartlett; H Thadepalli; A B Onderdonk
Journal:  J Clin Invest       Date:  1976-02       Impact factor: 14.808

8.  Effects of time and growth media on short-chain fatty acid production by Bacteroides fragilis.

Authors:  J W Mayhew; A B Onderdonk; S L Gorbach
Journal:  Appl Microbiol       Date:  1975-04

9.  Comparison of macroscopic examination, routine gram stains, and routine subcultures in the initial detection of positive blood cultures.

Authors:  D J Blazevic; J E Stemper; J M Matsen
Journal:  Appl Microbiol       Date:  1974-03

10.  Rapid diagnosis of anaerobic infections by gas-liquid chromatography.

Authors:  S Ladas; G Arapakis; H Malamou-Ladas; G Palikaris; A Arseni
Journal:  J Clin Pathol       Date:  1979-11       Impact factor: 3.411

  10 in total
  5 in total

1.  Clinical evaluation of diagnostic hemoperfusion for in vivo enrichment of bacteria and fungi in comparison with a conventional blood culture technique.

Authors:  E Kühnen; K P Schaal; F Keller; F Bartels
Journal:  J Clin Microbiol       Date:  1988-08       Impact factor: 5.948

2.  Diagnosis of bacteraemia by automated head-space capillary gas chromatography.

Authors:  L Larsson; P A Mårdh; G Odham; M L Carlsson
Journal:  J Clin Pathol       Date:  1982-07       Impact factor: 3.411

3.  Can direct gas-liquid chromatography of clinical samples detect specific organisms?

Authors:  B Watt; P A Geddes; O A Greenan; S K Napier; A Mitchell
Journal:  J Clin Pathol       Date:  1982-07       Impact factor: 3.411

4.  Gas-liquid chromatography of positive blood cultures for rapid presumptive diagnosis of anaerobic bacteremia.

Authors:  R S Edson; J E Rosenblatt; J A Washington; J B Stewart
Journal:  J Clin Microbiol       Date:  1982-06       Impact factor: 5.948

5.  Acridine orange stain in the early detection of bacteria in blood cultures.

Authors:  M Meseguer; L de Rafael; M Baquero; M Martínez Ferrer; M López-Brea
Journal:  Eur J Clin Microbiol       Date:  1984-04       Impact factor: 3.267

  5 in total

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