Literature DB >> 701376

Localization of cellular antigens in sodium dodecyl sulfate-polyacrylamide gels.

W S Adair, D Jurivich, U W Goodenough.   

Abstract

A procedure is described for localizing antigen-antibody complexes in sodium dodecyl sulfate (SDS) polyacrylamide gels using 125I-labeled protein A from Staphylococcus aureus. We use the procedure to probe antigenic cross-reactivities between Strongylocentrotus and Chlamydomonas alpha- and beta-tubulins; we also domonstrate how the procedure can detect minor antibody species in an antiserum directed against a cell membrane.

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Year:  1978        PMID： 701376      PMCID： PMC2110225          DOI： 10.1083/jcb.79.1.281

Source DB:  PubMed          Journal:  J Cell Biol        ISSN： 0021-9525            Impact factor:   10.539


  9 in total

1.  Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.

Authors:  S W Kessler
Journal:  J Immunol       Date:  1975-12       Impact factor: 5.422

2.  THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.

Authors:  F C GREENWOOD; W M HUNTER; J S GLOVER
Journal:  Biochem J       Date:  1963-10       Impact factor: 3.857

3.  A comparison of membrane proteins of normal and transformed cells by lactoperoxidase labeling.

Authors:  N M Hogg
Journal:  Proc Natl Acad Sci U S A       Date:  1974-02       Impact factor: 11.205

4.  Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.

Authors:  G Fairbanks; T L Steck; D F Wallach
Journal:  Biochemistry       Date:  1971-06-22       Impact factor: 3.162

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  A new staining technique for proteins in polyacrylamide gels using coomassie brilliant blue G250.

Authors:  R W Blakesley; J A Boezi
Journal:  Anal Biochem       Date:  1977-10       Impact factor: 3.365

7.  Phylogenetic insight into evolution of mammalian Fc fragment of gamma G globulin using staphylococcal protein A.

Authors:  G Kronvall; U S Seal; J Finstad; R C Williams
Journal:  J Immunol       Date:  1970-01       Impact factor: 5.422

8.  Flagellar doublet microtubules: fractionation of minor components and alpha-tubulin from specific regions of the A-tubule.

Authors:  R W Linck
Journal:  J Cell Sci       Date:  1976-03       Impact factor: 5.285

9.  Chlamydomonas flagella. I. Isolation and electrophoretic analysis of microtubules, matrix, membranes, and mastigonemes.

Authors:  G B Witman; K Carlson; J Berliner; J L Rosenbaum
Journal:  J Cell Biol       Date:  1972-09       Impact factor: 10.539
  9 in total
  27 in total

1.  A human protein related to the major envelope protein of murine mammary tumor virus: identification and characterization.

Authors:  A S Dion; D C Farwell; A A Pomenti; A J Girardi
Journal:  Proc Natl Acad Sci U S A       Date:  1980-03       Impact factor: 11.205

2.  Characterization of a cellulose-binding, cellulase-containing complex in Clostridium thermocellum.

Authors:  R Lamed; E Setter; E A Bayer
Journal:  J Bacteriol       Date:  1983-11       Impact factor: 3.490

3.  Localization of prostaglandin D2 binding protein and NADP-linked 15-hydroxyprostaglandin D2 dehydrogenase in the Purkinje cells of miniature pig cerebellum.

Authors:  Y Watanabe; A Yamashita; H Tokumoto; O Hayaishi
Journal:  Proc Natl Acad Sci U S A       Date:  1983-07       Impact factor: 11.205

4.  Localization of lipophosphonoglycan in membranes of Acanthamoeba by using specific antibodies.

Authors:  C F Bailey; B Bowers
Journal:  Mol Cell Biol       Date:  1981-04       Impact factor: 4.272

5.  Immunofluorescence localization at the mammalian neuromuscular junction of the Mr 43,000 protein of Torpedo postsynaptic membranes.

Authors:  S C Froehner; V Gulbrandsen; C Hyman; A Y Jeng; R R Neubig; J B Cohen
Journal:  Proc Natl Acad Sci U S A       Date:  1981-08       Impact factor: 11.205

6.  Immunologic recognition of cell surface antigens in normal mouse neural tissues and in neuroepithelial cells of the OTT-6050 mouse teratoma. A radiometric, gel electrophoretic and morphologic (immunofluorescence and immunoperoxidase) study.

Authors:  P B Ramsay; S R VandenBerg; L F Eng; M M Herman; L J Rubinstein
Journal:  Acta Neuropathol       Date:  1982       Impact factor: 17.088

7.  A quantitative dot-immunobinding assay for proteins using nitrocellulose membrane filters.

Authors:  R Jahn; W Schiebler; P Greengard
Journal:  Proc Natl Acad Sci U S A       Date:  1984-03       Impact factor: 11.205

8.  Widespread distribution of protein I in the central and peripheral nervous systems.

Authors:  P De Camilli; T Ueda; F E Bloom; E Battenberg; P Greengard
Journal:  Proc Natl Acad Sci U S A       Date:  1979-11       Impact factor: 11.205

9.  Cellular and subcellular localization of protein I in the peripheral nervous system.

Authors:  G Fried; E J Nestler; P De Camilli; L Stjärne; L Olson; J M Lundberg; T Hökfelt; C C Ouimet; P Greengard
Journal:  Proc Natl Acad Sci U S A       Date:  1982-04       Impact factor: 11.205

10.  The cytoskeleton of digitonin-treated rat hepatocytes.

Authors:  G Fiskum; S W Craig; G L Decker; A L Lehninger
Journal:  Proc Natl Acad Sci U S A       Date:  1980-06       Impact factor: 11.205
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