Localization of prostaglandin D2 binding protein and NADP-linked 15-hydroxyprostaglandin D2 dehydrogenase in the Purkinje cells of miniature pig cerebellum.

High-affinity binding sites specific for prostaglandin D2 were visualized by the autoradiographical technique assisted by computerized image processing and color coding. In the cerebellum of miniature pig, high density of the binding was observed in the cortex, mainly in the Purkinje cell layer. Immunohistochemical studies on the cellular localization of NADP-linked 15-hydroxyprostaglandin D2 dehydrogenase were performed with the same cerebellum and revealed the presence of this enzyme also in the Purkinje cells. These findings imply that prostaglandin D2 binds to the Purkinje cells and, after its action, is degraded in situ by the specific degradative enzyme.