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Literature DB >> 7000120

Flow cytometric applications to tumour biology: prospects and pitfalls.

M R Raju, T S Johnson, N Tokita, E L Gillette.

Abstract

A brief review of cytometry instrumentation and its potential applications in tumour biology is presented using our recent data. DNA distribution measurements of cells from spontaneous dog tumours and cultured cells after exposure to X-rays, alpha particles or adriamycin are shown. The data show that DNA fluorescence measurments have application in the study of cell kinetics after either radiation or drug treatment. Extensive and careful experimentation is needed, however, to utilize the sophisticated developments in flow cytometry instrumentation.

Entities: Chemical Disease Species

Mesh：

Substances：

Year: 1980 PMID： 7000120 PMCID： PMC2149201

Source DB: PubMed Journal: Br J Cancer Suppl ISSN： 0306-9443

16 in total

1. A flow-system multiangle light-scattering instrument for cell characterization.

Authors: G C Salzman; J M Crowell; C A Goad; K M Hansen; R D Hiebert; P M LaBauve; J C Martin; M L Ingram; P F Mullaney
Journal: Clin Chem Date: 1975-08 Impact factor: 8.327

2. A bromodeoxyuridine (BUdR)-mithramycin technique for detecting cycling and non-cycling cells by flow microfluorometry.

Authors: D E Swartzendruber
Journal: Exp Cell Res Date: 1977-10-15 Impact factor: 3.905

Flow cytometric applications to tumour biology: prospects and pitfalls.

1. A flow-system multiangle light-scattering instrument for cell characterization.

2. A bromodeoxyuridine (BUdR)-mithramycin technique for detecting cycling and non-cycling cells by flow microfluorometry.

3. DNA histogram analysis of human hemopoietic cells.

4. Laser flow microphotometry for rapid analysis and sorting of mammalian cells.

5. Cell-cycle analysis in 20 minutes.

6. Recognition of cells in mitosis by flow cytofluormetry.

7. Analysis and sorting of living cells according to deoxyribonucleic acid content.

8. The influence of nuclear size and chromosome complement on prognosis of carcinoma of the cervix.

9. Radiation response of multicell spheroids--an in vitro tumour model.

10. Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining.

1. DNA flow cytometric measurements in inflammatory and malignant human gastric lesions.

2. ICRF 159-induced cell-cycle perturbation in vitro: its relationship to inhibition of colony-forming ability.