Literature DB >> 49354

Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining.

A Krishan.   

Abstract

A rapid method for the flow microfluorometric determination of the DNA content per cell is described. Incubation of cells in a hypotonic solution of propidium iodide results in disruption of the cell membrane and rapid staining of nuclear chromatin. DNA distribution histograms generated from cells stained by this method are identical to those generated after fixation and RNase digestion. In contrast to some earlier described methods, the present technique is rapid (5 min of processing), requires a minimal amount of material, and avoids formation of cell clumps.

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Year:  1975        PMID： 49354      PMCID： PMC2109516          DOI： 10.1083/jcb.66.1.188

Source DB:  PubMed          Journal:  J Cell Biol        ISSN： 0021-9525            Impact factor:   10.539


  8 in total

1.  Adaptation of the fluorescent Feulgen reaction to cells in suspension for flow microfluorometry.

Authors:  T T Trujillo; M A Van Dilla
Journal:  Acta Cytol       Date:  1972 Jan-Feb       Impact factor: 2.319

2.  Use of flow microfluorometry in detailed analysis of effects of chemical agents on cell cycle progression.

Authors:  R A Tobey; H A Crissman
Journal:  Cancer Res       Date:  1972-12       Impact factor: 12.701

3.  Microfluorimetric evaluation of cell kill kinetics with 1-beta-D-arabinofuranosylcytosine.

Authors:  X Yataganas; A Strife; A Perez; B D Clarkson
Journal:  Cancer Res       Date:  1974-10       Impact factor: 12.701

4.  2,3-Dihydro-1H-imidazo(1,2-b)pyrazole: a new inhibitor of deoxyribonucleic acid synthesis.

Authors:  H L Ennis; L Möller; J J Wang; O S Selawry
Journal:  Biochem Pharmacol       Date:  1971-10       Impact factor: 5.858

5.  Cell microfluorometry: a method for rapid fluorescence measurement.

Authors:  M A Van Dilla; T T Trujillo; P F Mullaney; J R Coulter
Journal:  Science       Date:  1969-03-14       Impact factor: 47.728

6.  A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization.

Authors:  J B LePecq; C Paoletti
Journal:  J Mol Biol       Date:  1967-07-14       Impact factor: 5.469

7.  The use of an ethidium analogue in the dye-buoyant density procedure for the isolation of closed circular DNA: the variation of the superhelix density of mitochondrial DNA.

Authors:  B Hudson; W B Upholt; J Devinny; J Vinograd
Journal:  Proc Natl Acad Sci U S A       Date:  1969-03       Impact factor: 11.205

8.  Rapid, simultaneous measurement of DNA, protein, and cell volume in single cells from large mammalian cell populations.

Authors:  H A Crissman; J A Steinkamp
Journal:  J Cell Biol       Date:  1973-12       Impact factor: 10.539
  8 in total
  389 in total

1.  Nuclear domains enriched in RNA 3'-processing factors associate with coiled bodies and histone genes in a cell cycle-dependent manner.

Authors:  W Schul; I van Der Kraan; A G Matera; R van Driel; L de Jong
Journal:  Mol Biol Cell       Date:  1999-11       Impact factor: 4.138

2.  Suppression of E1A-mediated transformation by the p50E4F transcription factor.

Authors:  E R Fernandes; R J Rooney
Journal:  Mol Cell Biol       Date:  1999-07       Impact factor: 4.272

3.  Enhancer-dependent transcriptional oscillations in mouse erythroleukemia cells.

Authors:  Y Q Feng; R Alami; E E Bouhassira
Journal:  Mol Cell Biol       Date:  1999-07       Impact factor: 4.272

4.  Lack of synchrony among multiple nuclei induces partial DNA fragmentation in V79 cells polyploidized by demecolcine.

Authors:  K Fujikawa-Yamamoto; C Ohdoi; H Yamagishi; Z P Zong; M Murakami; N Yamaguchi
Journal:  Cell Prolif       Date:  1999-12       Impact factor: 6.831

5.  Establishment of a tetraploid Meth-A cell line through polyploidization by demecolcine but not by staurosporine, K-252A and paclitaxel.

Authors:  K Fujikawa-Yamamoto; S Wang; H Yamagishi; C Ohdoi; H Murano; T Ikeda
Journal:  Cell Prolif       Date:  2001-08       Impact factor: 6.831

6.  Neural, not gonadal, origin of brain sex differences in a gynandromorphic finch.

Authors:  Robert J Agate; William Grisham; Juli Wade; Suzanne Mann; John Wingfield; Carolyn Schanen; Aarno Palotie; Arthur P Arnold
Journal:  Proc Natl Acad Sci U S A       Date:  2003-04-02       Impact factor: 11.205

7.  Octaploid Meth-A cells are established from a highly polyploidized cell population.

Authors:  Kohzaburo Fujikawa-Yamamoto; Hiroko Yamagishi; Minoru Miyagoshi
Journal:  Cell Prolif       Date:  2003-04       Impact factor: 6.831

8.  Establishment of a triploid V79 cell line from tetraploid cells obtained through polyploidization using K-252a.

Authors:  Kohzaburo Fujikawa-Yamamoto; Hiroko Yamagishi; Minoru Miyagoshi
Journal:  Cell Prolif       Date:  2002-12       Impact factor: 6.831

9.  Polygamain, a new microtubule depolymerizing agent that occupies a unique pharmacophore in the colchicine site.

Authors:  R M Hartley; J Peng; G A Fest; S Dakshanamurthy; D E Frantz; M L Brown; S L Mooberry
Journal:  Mol Pharmacol       Date:  2011-12-14       Impact factor: 4.436

10.  Niacin restriction upregulates NADPH oxidase and reactive oxygen species (ROS) in human keratinocytes.

Authors:  Claudia A Benavente; Elaine L Jacobson
Journal:  Free Radic Biol Med       Date:  2007-10-17       Impact factor: 7.376
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