Literature DB >> 4133816

Cell-cycle analysis in 20 minutes.

H A Crissman, R A Tobey.   

Abstract

Mithramycin added to mammalian cells fixed in aqueous ethanol is bound to DNA and fluoresces in direct proportion to the cellular DNA content. Quantitative fluorescence measurement by means of a high-speed flow system instrument provides a rapid method for cell-cycle analysis and for the first time permits continuous monitoring of cell-cycle kinetics during ongoing experiments.

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Year:  1974        PMID： 4133816     DOI： 10.1126/science.184.4143.1297

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  70 in total

1.  Assembly and secretion of pentameric IgM in a fusion between a nonsecreting B cell lymphoma and an IgG-secreting plasmacytoma.

Authors:  W C Raschke; E L Mather; M E Koshland
Journal:  Proc Natl Acad Sci U S A       Date:  1979-07       Impact factor: 11.205

2.  G1 arrest and down-regulation of cyclin E/cyclin-dependent kinase 2 by the protein kinase inhibitor staurosporine are dependent on the retinoblastoma protein in the bladder carcinoma cell line 5637.

Authors:  J B Schnier; K Nishi; D W Goodrich; E M Bradbury
Journal:  Proc Natl Acad Sci U S A       Date:  1996-06-11       Impact factor: 11.205

3.  Hormonal control of adrenocortical cell proliferation. Desensitization to ACTH and interaction between ACTH and fibroblast growth factor in bovine adrenocortical cell cultures.

Authors:  P J Hornsby; G N Gill
Journal:  J Clin Invest       Date:  1977-08       Impact factor: 14.808

4.  Variation of DNA polymerases-alpha, -beta. and -gamma during perinatal tissue growth and differentiation.

Authors:  U Hübscher; C C Kuenzle; S Spadari
Journal:  Nucleic Acids Res       Date:  1977-08       Impact factor: 16.971

5.  Extended application of flow microfluorometry by means of dual laser excitation.

Authors:  M Stöhr; H Eipel; K Goerttler; M Vogt-Schaden
Journal:  Histochemistry       Date:  1977-04-04

6.  The intra-nucleolar localization of amplified rDNA in Xenopus laevis oocytes.

Authors:  C H Thiébaud
Journal:  Chromosoma       Date:  1979-06-21       Impact factor: 4.316

7.  The effect of desferrioxamine on transferrin receptors, the cell cycle and growth rates of human leukaemic cells.

Authors:  A Bomford; J Isaac; S Roberts; A Edwards; S Young; R Williams
Journal:  Biochem J       Date:  1986-05-15       Impact factor: 3.857

8.  Rapid nuclear staining method for Saccharomyces cerevisiae.

Authors:  M L Slater
Journal:  J Bacteriol       Date:  1976-06       Impact factor: 3.490

9.  Interferon-directed inhibition of chronic murine leukemia virus production in cell cultures: lack of effect on intracellular viral markers.

Authors:  R M Friedman; E H Chang; J M Ramseur; M W Myers
Journal:  J Virol       Date:  1975-09       Impact factor: 5.103

10.  Multiple kinase arrest points in the G1 phase of nontransformed mammalian cells are absent in transformed cells.

Authors:  D M Gadbois; H A Crissman; R A Tobey; E M Bradbury
Journal:  Proc Natl Acad Sci U S A       Date:  1992-09-15       Impact factor: 11.205
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