Literature DB >> 6997242

Immunohistochemistry of brain 5-hydroxytryptamine.

R Buffa, O Crivelli, C Lavarini, F Sessa, G Verme, E Solcia.   

Abstract

An immunohistochemical assay for 5-hydroxytryptamine (5HT) has been developed, validated by parallel radioimmunoassay and a series of tests with monoamines or related molecules, and applied to the detection of 5HT in rat brain sections. The procedure seems to be more sensitive and specific than the classical Falck-Hillarp technique. Among amines and related compounds tested, only 5-methoxytryptamine has been found to cross-react. 5HT-immunoreactive neurons and/or fibres have been observed in the spinal cord, brain stem, hypothalamic nuclei, epiphysis and subcommissural organ, thalamus, striatum, corpus callosum, amygdala, hippocampus, olfactory tubercle, and cerebral cortex.

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Year:  1980        PMID： 6997242     DOI： 10.1007/bf00498495

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  9 in total

1.  Aqueous aldehyde (Faglu) methods for the fluorescence histochemical localization of catecholamines and for ultrastructural studies of central nervous tissue.

Authors:  J B Furness; J W Heath; M Costa
Journal:  Histochemistry       Date:  1978-09-28

2.  Purification and properties of hydroxyindole-O-methyl transferase.

Authors:  J AXELROD; H WEISSBACH
Journal:  J Biol Chem       Date:  1961-01       Impact factor: 5.157

3.  EVIDENCE FOR THE EXISTENCE OF MONOAMINE NEURONS IN THE CENTRAL NERVOUS SYSTEM. 3. THE MONOAMINE NERVE TERMINAL.

Authors:  K FUXE
Journal:  Z Zellforsch Mikrosk Anat       Date:  1965-02-09

4.  Complement-mediated unspecific binding of immunoglobulins to some endocrine cells.

Authors:  R Buffa; O Crivelli; R Fiocca; P Fontana; E Solcia
Journal:  Histochemistry       Date:  1979-09

5.  Immunocytochemical demonstration of 5-hydroxytryptamine in gastrointestinal endocrine cells.

Authors:  P Facer; J M Polak; B M Jaffe; A G Pearse
Journal:  Histochem J       Date:  1979-01

Review 6.  Localization of indolealkylamines in CNS.

Authors:  K Fuxe; T Hökfelt; U Ungerstedt
Journal:  Adv Pharmacol       Date:  1968

7.  Validation and application of a radioimmunoassay for serotonin.

Authors:  J M Kellum; B M Jaffe
Journal:  Gastroenterology       Date:  1976-04       Impact factor: 22.682

8.  Serotonin: radioimmunoassay.

Authors:  B Peskar; S Spector
Journal:  Science       Date:  1973-03-30       Impact factor: 47.728

9.  Immunohistochemical identification of the cholecystokinin cell in the intestinal mucosa.

Authors:  R Buffa; E Solcia; V L Go
Journal:  Gastroenterology       Date:  1976-04       Impact factor: 22.682
  9 in total
  3 in total

1.  Immunohistochemical demonstration of EC cells in rat gastrointestinal tract.

Authors:  H Inokuchi; K Kawai; Y Takeuchi; Y Sano
Journal:  Histochemistry       Date:  1982

2.  Immunohistochemical demonstration of the distribution of serotonin neurons in the brainstem of the rat and cat.

Authors:  Y Takeuchi; H Kimura; Y Sano
Journal:  Cell Tissue Res       Date:  1982       Impact factor: 5.249

3.  Anti-5-hydroxytryptamine antibodies: studies on their cross-reactivity in vitro and their immunohistochemical specificity.

Authors:  H Bras; G Chazal; J Destombes; J J Puizillout
Journal:  Exp Brain Res       Date:  1986       Impact factor: 1.972
  3 in total

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