Literature DB >> 372152

Immunocytochemical demonstration of 5-hydroxytryptamine in gastrointestinal endocrine cells.

P Facer, J M Polak, B M Jaffe, A G Pearse.   

Abstract

We have been able to demonstrate 5-hydroxytryptamine in the enterochromaffin cells of the mammalian gastrointestinal tract, using a highly specific antiserum. Conventional histochemical techniques for identifying amines as cell markers can thus be replaced by more reliable and sensitive immunocytochemical methods. This has been facilitated by the use of p-benzoquinone as fixative which has been shown to preserve the localization and antigenicity of amines, as well as peptides.

Entities:  

Mesh:

Substances:

Year:  1979        PMID: 372152     DOI: 10.1007/bf01041271

Source DB:  PubMed          Journal:  Histochem J        ISSN: 0018-2214


  6 in total

1.  Bifunctional reagents as vapour- and liquid-phase fixatives for immunohistochemistry.

Authors:  A G Pearse; J M Polak
Journal:  Histochem J       Date:  1975-03

2.  Comparative biochemistry of the phenolase complex.

Authors:  H S MASON
Journal:  Adv Enzymol Relat Subj Biochem       Date:  1955

3.  The reaction of benzoquinone with amines and proteins.

Authors:  M Morrison; W Steele; D J Danner
Journal:  Arch Biochem Biophys       Date:  1969-11       Impact factor: 4.013

4.  Validation and application of a radioimmunoassay for serotonin.

Authors:  J M Kellum; B M Jaffe
Journal:  Gastroenterology       Date:  1976-04       Impact factor: 22.682

5.  Immunoelectron cytochemical localization of motilin and substance P in rabbit bile duct enterochromaffin (EC) cells.

Authors:  P Heitz; J M Polak; M Kasper; C M Timson; A G Pearse
Journal:  Histochemistry       Date:  1977-02-01

6.  Studies on antibody production. I. A method for the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit.

Authors:  A H COONS; E H LEDUC; J M CONNOLLY
Journal:  J Exp Med       Date:  1955-07-01       Impact factor: 14.307

  6 in total
  18 in total

1.  Immunohistochemical localization of aromatic L-amino acid decarboxylase in mouse taste buds and developing taste papillae.

Authors:  Yuji Seta; Shinji Kataoka; Takashi Toyono; Kuniaki Toyoshima
Journal:  Histochem Cell Biol       Date:  2007-01-09       Impact factor: 4.304

2.  Mediation by 5_hydroxytryptamine of the femoral vasoconstriction induced by acid challenge of the rat gastric mucosa.

Authors:  C H Wachter; A Heinemann; J Donnerer; M A Pabst; P Holzer
Journal:  J Physiol       Date:  1998-06-01       Impact factor: 5.182

3.  Serotonin-containing epithelial cells in rat duodenum. I. Quantitative morphometric study of the distribution density.

Authors:  M Fujimiya; T Maeda; H Kimura
Journal:  Histochemistry       Date:  1991

4.  Serotonin-containing EC cells in normal human gastric mucosa and in gastritis. Immunohistochemical, electron microscopic and autoradiographic studies.

Authors:  H Ito; H Yokozaki; K Tokumo; S Nakajo; E Tahara
Journal:  Virchows Arch A Pathol Anat Histopathol       Date:  1986

5.  A novel subtyping of intestinal metaplasia of the stomach, with special reference to the histochemical characterizations of endocrine cells.

Authors:  Y Tsutsumi; H Nagura; K Watanabe; N Yanaihara
Journal:  Virchows Arch A Pathol Anat Histopathol       Date:  1983

6.  Identification of EC cells in the human intestine: a comparative study between immunohistochemical and silver impregnation techniques.

Authors:  H Inokuchi; K Kawai; Y Takeuchi; Y Sano
Journal:  Histochemistry       Date:  1983

7.  Immunohistochemical study on the morphology of enterochromaffin cells in the human fundic mucosa.

Authors:  H Inokuchi; K Kawai; Y Takeuchi; Y Sano
Journal:  Cell Tissue Res       Date:  1984       Impact factor: 5.249

8.  Serotonin immunohistochemistry reveals immature EC cells.

Authors:  H Inokuchi; T Azuma; K Kawai; Y Takeuchi; Y Sano
Journal:  Histochemistry       Date:  1984

9.  Anti-5-hydroxytryptamine antibodies: studies on their cross-reactivity in vitro and their immunohistochemical specificity.

Authors:  H Bras; G Chazal; J Destombes; J J Puizillout
Journal:  Exp Brain Res       Date:  1986       Impact factor: 1.972

10.  Serotonin-storing cells of the chicken duodenum: light, fluorescence and electron microscopy, and immunohistochemistry.

Authors:  T Watanabe; H Chikazawa; N Chungsamarnyart; T Fujioka; J Yamada
Journal:  Cell Tissue Res       Date:  1987-01       Impact factor: 5.249

View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.