Literature DB >> 6993344

Cell aggregate suspension culture for large-scale production of biomolecules.

W R Tolbert, M M Hitt, J Feder.   

Abstract

A method is described for the rapid reversible conversion of a number of continuous cell lines from anchorage-dependent growth to growth as aggregates of cells in suspension culture. Employing this technique, an inoculum of three 75-cm2 flasks of BALB/c SV3T3 cells was grown to 60 liters of aggregate suspension in 14 days. This yielded 120 ml of packed cells or 9.1 x 10(10) cells. Similar results were obtained for other cell lines. Biomolecules such as migration-inhibition factor (MIF) and plasminogen activator were produced from these cultures.

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Year:  1980        PMID： 6993344     DOI： 10.1007/bf02626461

Source DB:  PubMed          Journal:  In Vitro        ISSN： 0073-5655


  14 in total

1.  A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.

Authors:  K BURTON
Journal:  Biochem J       Date:  1956-02       Impact factor: 3.857

2.  Arrest of 3T3 cells in G1 phase in suspension culture.

Authors:  H Otsuka; M Moskowitz
Journal:  J Cell Physiol       Date:  1975-12       Impact factor: 6.384

3.  A simplified method for production and growth of multicellular tumor spheroids.

Authors:  J M Yuhas; A P Li; A O Martinez; A J Ladman
Journal:  Cancer Res       Date:  1977-10       Impact factor: 12.701

4.  Breast tumor cell lines from pleural effusions.

Authors:  R Cailleau; R Young; M Olivé; W J Reeves
Journal:  J Natl Cancer Inst       Date:  1974-09       Impact factor: 13.506

5.  Growth of multicell spheroids in tissue culture as a model of nodular carcinomas.

Authors:  R M Sutherland; J A McCredie; W R Inch
Journal:  J Natl Cancer Inst       Date:  1971-01       Impact factor: 13.506

6.  Transformation of mouse cell line 3T3 by SV40: dose response relationship and correlation with SV40 tumor antigen production.

Authors:  P H Black
Journal:  Virology       Date:  1966-04       Impact factor: 3.616

7.  Comparative growth characteristics of VERO cells on gas-permeable and conventional supports.

Authors:  M D Jensen; D F Wallach; P S Lin
Journal:  Exp Cell Res       Date:  1974-03-15       Impact factor: 3.905

8.  Autoclavable low cost serum-free cell culture media: the growth of established cell lines and production of viruses.

Authors:  L Keay
Journal:  Biotechnol Bioeng       Date:  1976-03       Impact factor: 4.530

9.  In vitro analysis of the response of multicellular tumor spheroids exposed to chemotherapeutic agents in vitro or in vivo.

Authors:  J M Yuhas; A E Tarleton; J G Harman
Journal:  Cancer Res       Date:  1978-11       Impact factor: 12.701

10.  Multicellular spheroids: a new model target for in vitro studies of immunity to solid tumor allografts.

Authors:  R M Sutherland; H R MacDonald; R L Howell
Journal:  J Natl Cancer Inst       Date:  1977-06       Impact factor: 13.506
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  10 in total

1.  The growth of Vero cells in suspension as cell-aggregates in serum-free media.

Authors:  J Litwin
Journal:  Cytotechnology       Date:  1992       Impact factor: 2.058

2.  Changes in animal cell natural aggregates in suspended batch cultures.

Authors:  J L Moreira; P M Alves; J G Aunins; M J Carrondo
Journal:  Appl Microbiol Biotechnol       Date:  1994-04       Impact factor: 4.813

3.  Expression of recombinant antibody and secreted alkaline phosphatase in mammalian cells. Influence of cell line and culture system upon production kinetics.

Authors:  A J Racher; J L Moreira; P M Alves; M Wirth; U H Weidle; H Hauser; M J Carrondo; J B Griffiths
Journal:  Appl Microbiol Biotechnol       Date:  1994-02       Impact factor: 4.813

4.  An assay measuring the stimulation of several types of bovine endothelial cells by growth factor(s) derived from cultured human tumor cells.

Authors:  J V Olander; J C Marasa; R C Kimes; G M Johnston; J Feder
Journal:  In Vitro       Date:  1982-02

5.  Mucin-coating technologies for protection and reduced aggregation of cellular production systems.

Authors:  Carolyn R Shurer; Shelby E Head; Marc C Goudge; Matthew J Paszek
Journal:  Biotechnol Bioeng       Date:  2019-02-19       Impact factor: 4.530

6.  Evaluation of anchorage-dependent cell propagation systems for production of human acetylcholinesterase by recombinant 293 cells.

Authors:  A Lazar; S Reuveny; C Kronman; B Velan; A Shafferman
Journal:  Cytotechnology       Date:  1993       Impact factor: 2.058

7.  Repeated-batch cultures of baby hamster kidney cell aggregates in stirred vessels.

Authors:  J L Moreira; A S Feliciano; P C Santana; P E Cruz; J G Aunins; M J Carrondo
Journal:  Cytotechnology       Date:  1994       Impact factor: 2.058

8.  Cultivation of anchorage-dependent animal cells in microsphere-induced aggregate culture.

Authors:  S Goetghebeur; W S Hu
Journal:  Appl Microbiol Biotechnol       Date:  1991-03       Impact factor: 4.813

9.  Production of a membrane-bound protein, the human gamma-glutamyl transferase, by CHO cells cultivated on microcarriers, in aggregates and in suspension.

Authors:  I Chevalot; A Visvikis; P Nabet; J M Engasser; A Marc
Journal:  Cytotechnology       Date:  1994       Impact factor: 2.058

10.  Single-Cell Tracking of Breast Cancer Cells Enables Prediction of Sphere Formation from Early Cell Divisions.

Authors:  Patrick C Bailey; Rachel M Lee; Michele I Vitolo; Stephen J P Pratt; Eleanor Ory; Kristi Chakrabarti; Cornell J Lee; Keyata N Thompson; Stuart S Martin
Journal:  iScience       Date:  2018-08-21
  10 in total

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