Literature DB >> 1369212

The growth of Vero cells in suspension as cell-aggregates in serum-free media.

J Litwin1.   

Abstract

Vero cell lines, usually considered anchorage-dependent, could be grown as cell-aggregates in suspension culture with serum-free media. Several different combinations of base media gave growth results above 10(6) cells/ml (NCTC 135:SFRE 199-1; NCTC 135:Waymouth MB 752/1; NCTC 135:RPMI 1640). Insulin was not essential for growth and Bovine Serum Albumin could be diluted out of the media if linoleic acid was present. The size and density of the aggregates formed varied depending on the media used.

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Year:  1992        PMID： 1369212     DOI： 10.1007/bf00570893

Source DB:  PubMed          Journal:  Cytotechnology        ISSN： 0920-9069            Impact factor:   2.058


  6 in total

1.  Growth of Vero E-6 cells on microcarriers in a cell bioreactor.

Authors:  L A White; E W Ades
Journal:  J Clin Microbiol       Date:  1990-02       Impact factor: 5.948

Review 2.  Multicell spheroids as a model for cell kinetic studies.

Authors:  R E Durand
Journal:  Cell Tissue Kinet       Date:  1990-05

Review 3.  Multicellular tumor spheroids in radiotherapy research (review).

Authors:  J H Schwachöfer
Journal:  Anticancer Res       Date:  1990 Jul-Aug       Impact factor: 2.480

Review 4.  Influence of geometry on control of cell growth.

Authors:  J Folkman; H P Greenspan
Journal:  Biochim Biophys Acta       Date:  1975-12-31

5.  Growth of poliovirus using alginate entrapped-anchorage dependent Vero cells.

Authors:  P Trépanier; R Dugré; R Alain; J Lapierre
Journal:  Cytotechnology       Date:  1990-11       Impact factor: 2.058

6.  Cell aggregate suspension culture for large-scale production of biomolecules.

Authors:  W R Tolbert; M M Hitt; J Feder
Journal:  In Vitro       Date:  1980-06
  6 in total
  8 in total

1.  The role of recombinant proteins in the development of serum-free media.

Authors:  Joanne Keenan; Dermot Pearson; Martin Clynes
Journal:  Cytotechnology       Date:  2006-08-05       Impact factor: 2.058

2.  The new medium MDSS2N, free of any animal protein supports cell growth and production of various viruses.

Authors:  O W Merten; H Kallel; J C Manuguerra; M Tardy-Panit; R Crainic; F Delpeyroux; S Van der Werf; P Perrin
Journal:  Cytotechnology       Date:  1999-07       Impact factor: 2.058

3.  Evaluation of the new serum-free medium (MDSS2) for the production of different biologicals: use of various cell lines.

Authors:  O W Merten; J V Kierulff; N Castignolles; P Perrin
Journal:  Cytotechnology       Date:  1994       Impact factor: 2.058

4.  Engineering Vero cells to secrete human insulin.

Authors:  Lorraine O'Driscoll; Patrick Gammell; Martin Clynes
Journal:  In Vitro Cell Dev Biol Anim       Date:  2002-03       Impact factor: 2.416

5.  Repeated-batch cultures of baby hamster kidney cell aggregates in stirred vessels.

Authors:  J L Moreira; A S Feliciano; P C Santana; P E Cruz; J G Aunins; M J Carrondo
Journal:  Cytotechnology       Date:  1994       Impact factor: 2.058

6.  A serum-free Vero production platform for a chimeric virus vaccine candidate.

Authors:  Inn H Yuk; Gina B Lin; Hui Ju; Inesse Sifi; Yvonne Lam; Armida Cortez; Danny Liebertz; J Michael Berry; Richard M Schwartz
Journal:  Cytotechnology       Date:  2006-11-16       Impact factor: 2.058

7.  Evaluation of recombinant human transferrin (DeltaFerrin(TM)) as an iron chelator in serum-free media for mammalian cell culture.

Authors:  Joanne Keenan; Dermot Pearson; Lorraine O'Driscoll; Patrick Gammell; Martin Clynes
Journal:  Cytotechnology       Date:  2006-08-03       Impact factor: 2.058

8.  Vero cells gain renal tubule markers in low-calcium and magnesium chemically defined media.

Authors:  Megan Logan; Karsten Rinas; Brendan McConkey; Marc G Aucoin
Journal:  Sci Rep       Date:  2022-04-13       Impact factor: 4.379
  8 in total

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