Literature DB >> 6314329

Genomic cloning and preliminary characterization of the human thymidine kinase gene.

P F Lin, S Y Zhao, F H Ruddle.   

Abstract

In this report, we describe the cloning of the human cytoplasmic thymidine kinase (tk-C; EC 2.7.1.21) gene and its preliminary characterization. The tk-C sequences were isolated from a phage genomic library made from DNA of a transfected mouse cell carrying the human tk-C gene. The human transforming sequences were identified by homology with human Alu sequences. Six recombinant phages were isolated and five were competent to transfer human TK-C activity to TK-deficient mouse cells when transferred in pairs. Conclusively, sequences homologous to these clones are present in all human TK+ transformants examined. We estimate the maximal size of the tk-C gene to be 14 kilobase pairs and its minimal size to be between 4 and 5 kilobase pairs. The gene contains many noncoding inserts and numerous Alu sequences.

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Year:  1983        PMID： 6314329      PMCID： PMC390386          DOI： 10.1073/pnas.80.21.6528

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  23 in total

1.  Chemical suppression of the immune response.

Authors:  G H HITCHINGS; G B ELION
Journal:  Pharmacol Rev       Date:  1963-06       Impact factor: 25.468

2.  Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells.

Authors:  M Wigler; S Silverstein; L S Lee; A Pellicer; Y c Cheng; R Axel
Journal:  Cell       Date:  1977-05       Impact factor: 41.582

3.  Construction, propagation and expression of simian virus 40 recombinant genomes containing the Escherichia coli gene for thymidine kinase and a Saccharomyces cerevisae gene for tyrosine transfer RNA.

Authors:  S P Goff; P Berg
Journal:  J Mol Biol       Date:  1979-09-25       Impact factor: 5.469

4.  Construction and characterization of a recombinant plasmid encoding the gene for the thymidine kinase of Herpes simplex type 1 virus.

Authors:  L W Enquist; G F Vande Woude; M Wagner; J R Smiley; W C Summers
Journal:  Gene       Date:  1979-11       Impact factor: 3.688

5.  Thymidine kinase, DNA synthesis and cancer.

Authors:  S Kit
Journal:  Mol Cell Biochem       Date:  1976-06-15       Impact factor: 3.396

6.  Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor.

Authors:  M Wigler; A Pellicer; S Silverstein; R Axel
Journal:  Cell       Date:  1978-07       Impact factor: 41.582

7.  Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.

Authors:  G M Wahl; M Stern; G R Stark
Journal:  Proc Natl Acad Sci U S A       Date:  1979-08       Impact factor: 11.205

8.  Screening lambdagt recombinant clones by hybridization to single plaques in situ.

Authors:  W D Benton; R W Davis
Journal:  Science       Date:  1977-04-08       Impact factor: 47.728

9.  Transfer of genetic information by purified metaphase chromosomes.

Authors:  O W McBride; H L Ozer
Journal:  Proc Natl Acad Sci U S A       Date:  1973-04       Impact factor: 11.205

10.  Human mitochondrial thymidine kinase is coded for by a gene on chromosome 16 of the nucleus.

Authors:  K Willecke; T Teber; R S Kucherlapati; F H Ruddle
Journal:  Somatic Cell Genet       Date:  1977-05
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  18 in total

1.  Partial complementation of the Fanconi anemia defect upon transfection by heterologous DNA. Phenotypic dissociation of chromosomal and cellular hypersensitivity to DNA cross-linking agents.

Authors:  C Diatloff-Zito; F Rosselli; J Heddle; E Moustacchi
Journal:  Hum Genet       Date:  1990-12       Impact factor: 4.132

2.  Identification of DNA sequences required for mouse APRT gene expression.

Authors:  M K Dush; M R Briggs; M E Royce; D A Schaff; S A Khan; J A Tischfield; P J Stambrook
Journal:  Nucleic Acids Res       Date:  1988-09-12       Impact factor: 16.971

3.  S-phase-specific regulation by deletion mutants of the human thymidine kinase promoter.

Authors:  K E Lipson; S T Chen; J Koniecki; D H Ku; R Baserga
Journal:  Proc Natl Acad Sci U S A       Date:  1989-09       Impact factor: 11.205

4.  Induction of cellular thymidine kinase occurs at the mRNA level.

Authors:  P Stuart; M Ito; C Stewart; S E Conrad
Journal:  Mol Cell Biol       Date:  1985-06       Impact factor: 4.272

5.  Genetic determinants of growth phase-dependent and adenovirus 5-responsive expression of the Chinese hamster thymidine kinase gene are contained within thymidine kinase mRNA sequences.

Authors:  J A Lewis; D A Matkovich
Journal:  Mol Cell Biol       Date:  1986-06       Impact factor: 4.272

6.  Structure and expression of the Chinese hamster thymidine kinase gene.

Authors:  J A Lewis
Journal:  Mol Cell Biol       Date:  1986-06       Impact factor: 4.272

7.  Isolation of the CD7 gene from the DNA of transfected L cells.

Authors:  K E Lovering; N J Deacon; C A Mickelson; H A Vaughan; K M Curnow; I F McKenzie; M S Sandrin
Journal:  Immunogenetics       Date:  1987       Impact factor: 2.846

8.  Genetic analysis of the human thymidine kinase gene promoter.

Authors:  J A Kreidberg; T J Kelly
Journal:  Mol Cell Biol       Date:  1986-08       Impact factor: 4.272

9.  Control of thymidine kinase mRNA during the cell cycle.

Authors:  D L Coppock; A B Pardee
Journal:  Mol Cell Biol       Date:  1987-08       Impact factor: 4.272

10.  Direct isolation of the functional human thymidine kinase gene with a cosmid shuttle vector.

Authors:  Y F Lau; Y W Kan
Journal:  Proc Natl Acad Sci U S A       Date:  1984-01       Impact factor: 11.205
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