Literature DB >> 6941243

Purification of thymus mRNA coding for a 16,000-dalton polypeptide containing the thymosin alpha 1 sequence.

M Freire, E Hannappel, M Rey, J M Freire, H Kido, B L Horecker.   

Abstract

A mRNA fraction purified by preparative polyacrylamide disc gel electrophoresis from calf thymus polysomes codes for a polypeptide(s) having a mass of 16,000-17,000 daltons. This polypeptide contains amino acid sequences corresponding to residues 11-18 and 19-25 of thymosin alpha 1. The yield of the octapeptide indicates that the 16,000-dalton peptide is the major product formed in the cell-free synthesis system containing the purified mRNA.

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Year:  1981        PMID： 6941243      PMCID： PMC319017          DOI： 10.1073/pnas.78.1.192

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  9 in total

1.  Automatic Monitoring of primary amines in preparative column effluents with fluorescamine.

Authors:  P Böhlen; S Stein; J Stone; S Udenfriend
Journal:  Anal Biochem       Date:  1975-08       Impact factor: 3.365

2.  The chemistry and biology of thymosin. I. Isolation, characterization, and biological activities of thymosin alpha1 and polypeptide beta1 from calf thymus.

Authors:  T L Low; G B Thurman; M McAdoo; J McClure; J L Rossio; P H Naylor; A L Goldstein
Journal:  J Biol Chem       Date:  1979-02-10       Impact factor: 5.157

3.  Cleavage at glutamic acid with staphylococcal protease.

Authors:  G R Drapeau
Journal:  Methods Enzymol       Date:  1977       Impact factor: 1.600

4.  Thymosin alpha1: isolation and sequence analysis of an immunologically active thymic polypeptide.

Authors:  A L Goldstein; T L Low; M McAdoo; J McClure; G B Thurman; J Rossio; C Y Lai; D Chang; S S Wang; C Harvey; A H Ramel; J Meienhofer
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

5.  Electrophoresis of RNA in formamide.

Authors:  J C Pinder; D Z Staynov; W B Gratzer
Journal:  Biochemistry       Date:  1974-12-17       Impact factor: 3.162

6.  Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid.

Authors:  R D Palmiter
Journal:  Biochemistry       Date:  1974-08-13       Impact factor: 3.162

7.  The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.

Authors:  U E Loening
Journal:  Biochem J       Date:  1967-01       Impact factor: 3.857

8.  Translation of mRNA from calf thymus in the wheat germ system: evidence for a precursor of thymosin alpha1.

Authors:  M Freire; O Crivellaro; C Isaacs; J Moschera; B L Horecker
Journal:  Proc Natl Acad Sci U S A       Date:  1978-12       Impact factor: 11.205

9.  Preparative high-performance liquid partition chromatography of proteins.

Authors:  M Rubinstein
Journal:  Anal Biochem       Date:  1979-09-15       Impact factor: 3.365
  9 in total
  2 in total

1.  Thymosin alpha 11: a peptide related to thymosin alpha 1 isolated from calf thymosin fraction 5.

Authors:  J Caldarella; G J Goodall; A M Felix; E P Heimer; S B Salvin; B L Horecker
Journal:  Proc Natl Acad Sci U S A       Date:  1983-12       Impact factor: 11.205

2.  Involution patterns of the human thymus. I Size of the cortical area as a function of age.

Authors:  P Tosi; R Kraft; P Luzi; M Cintorino; G Fankhauser; M W Hess; H Cottier
Journal:  Clin Exp Immunol       Date:  1982-02       Impact factor: 4.330
  2 in total

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