Literature DB >> 6935667

Use of an indicator sequence of human DNA to study DNA damage by methylbis(2-chloroethyl)amine.

S M Grunberg, W A Haseltine.   

Abstract

A highly reiterated sequence of human DNA was used to determine the sites of modification of DNA caused by the anti-tumor drug methylbis(2-chloroethyl)amine (HN2, mechlorethamine, nitrogen mustard) upon treatment of cells in culture and of purified DNA. The lengths of the breakage products of the DNA treated with HN2 were compared to the lengths of DNA scission products produced by chemical reactions used for DNA sequence determination. HN2 was found to create alkali-labile lesions at positions of guanine. The distribution of the guanine modifications was the same for DNA extracted from cells treated with HN2 and for purified DNA treated with HN2. However, the extent of damage was at least 2-fold greater when purified DNA was used as the substrate. Several nitrogen mustard analogues also produced alkali-labile lesions at positions of guanine.

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Year:  1980        PMID： 6935667      PMCID： PMC350322          DOI： 10.1073/pnas.77.11.6546

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  14 in total

1.  A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase.

Authors:  F Sanger; A R Coulson
Journal:  J Mol Biol       Date:  1975-05-25       Impact factor: 5.469

2.  Homology between human and simian repeated DNA.

Authors:  L Manuelidis; J C Wu
Journal:  Nature       Date:  1978-11-02       Impact factor: 49.962

3.  Specificity of substrate recognition by the EcoRI restriction endonuclease.

Authors:  B Polisky; P Greene; D E Garfin; B J McCarthy; H M Goodman; H W Boyer
Journal:  Proc Natl Acad Sci U S A       Date:  1975-09       Impact factor: 11.205

4.  Synthesis and structure-activity relationships of pre-activated analogs of cyclophosphamide (NSC-26271).

Authors:  J A Montgomery; R F Struck
Journal:  Cancer Treat Rep       Date:  1976-04

5.  A new method for sequencing DNA.

Authors:  A M Maxam; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

6.  Subunit structure of chromatin and the organization of eukaryotic highly repetitive DNA: recurrent periodicities and models for the evolutionary origins of repetitive DNA.

Authors:  J J Maio; F L Brown; P R Musich
Journal:  J Mol Biol       Date:  1977-12-15       Impact factor: 5.469

7.  Interstrand cross-linking of DNA by difunctional alkylating agents.

Authors:  P D Lawley; P Brookes
Journal:  J Mol Biol       Date:  1967-04-14       Impact factor: 5.469

Review 8.  The metabolic fate of cyclophosphamide.

Authors:  A R Torkelson; J A LaBudde; J H Weikel
Journal:  Drug Metab Rev       Date:  1974       Impact factor: 4.518

9.  Reaction of nucleosome DNA with dimethyl sulfate.

Authors:  J D McGhee; G Felsenfeld
Journal:  Proc Natl Acad Sci U S A       Date:  1979-05       Impact factor: 11.205

10.  Repeating restriction fragments of human DNA.

Authors:  L Manuelidis
Journal:  Nucleic Acids Res       Date:  1976-11       Impact factor: 16.971
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  12 in total

1.  Comparison of the sequence specificity of bleomycin cleavage in two slightly different DNA sequences.

Authors:  V Murray; R F Martin
Journal:  Nucleic Acids Res       Date:  1985-03-11       Impact factor: 16.971

2.  T4 DNA polymerase (3'-5') exonuclease, an enzyme for the detection and quantitation of stable DNA lesions: the ultraviolet light example.

Authors:  P W Doetsch; G L Chan; W A Haseltine
Journal:  Nucleic Acids Res       Date:  1985-05-10       Impact factor: 16.971

Review 3.  Sequence specificities in the interactions of chemicals and radiations with DNA.

Authors:  R J Wilkins
Journal:  Mol Cell Biochem       Date:  1984-09       Impact factor: 3.396

4.  Distribution of UV light-induced damage in a defined sequence of human DNA: detection of alkaline-sensitive lesions at pyrimidine nucleoside-cytidine sequences.

Authors:  J A Lippke; L K Gordon; D E Brash; W A Haseltine
Journal:  Proc Natl Acad Sci U S A       Date:  1981-06       Impact factor: 11.205

5.  DNA sequence selectivity of guanine-N7 alkylation by nitrogen mustards is preserved in intact cells.

Authors:  J A Hartley; J P Bingham; R L Souhami
Journal:  Nucleic Acids Res       Date:  1992-06-25       Impact factor: 16.971

6.  Removal of UV light-induced pyrimidine-pyrimidone(6-4) products from Escherichia coli DNA requires the uvrA, uvrB, and urvC gene products.

Authors:  W A Franklin; W A Haseltine
Journal:  Proc Natl Acad Sci U S A       Date:  1984-06       Impact factor: 11.205

7.  Influence of DNA repair defects (rad1, rad52) on nitrogen mustard mutagenesis in yeast.

Authors:  J R Mis; B A Kunz
Journal:  Mol Gen Genet       Date:  1992-11

8.  DNA lability induced by nimustine and ramustine in rat glioma cells.

Authors:  K Mineura; S Fushimi; Y Itoh; M Kowada
Journal:  J Neurol Neurosurg Psychiatry       Date:  1988-11       Impact factor: 10.154

9.  Use of exonuclease III to determine the site of stable lesions in defined sequences of DNA: the cyclobutane pyrimidine dimer and cis and trans dichlorodiammine platinum II examples.

Authors:  B Royer-Pokora; L K Gordon; W A Haseltine
Journal:  Nucleic Acids Res       Date:  1981-09-25       Impact factor: 16.971

10.  Reassessing the genotoxic potential of 8-MOP + UVA-induced DNA damage in the yeast Saccharomyces cerevisiae.

Authors:  J A Henriques; H H Andrade; M Bankmann; M Brendel
Journal:  Curr Genet       Date:  1989-08       Impact factor: 3.886
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