Literature DB >> 6929948

Length determination of the terminal redundant regions in the DNA of phage T7.

B Dreiseikelmann, U Steger, W Wackernagel.   

Abstract

The length of the terminal redundant regions in T7 DNA has been determined by two methods. One involved the specific labeling and isolation of the redundant DNA fragment and determination of the molecular weight by polyacrylamide gel electrophoresis. A value of 150 +/- 10 nucleotide pairs was obtained. The other determination based on a correlation of the melting temperature of the redundant region to that of whole T7 DNA confirmed the result obtained by the first method.

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Year:  1980        PMID: 6929948     DOI: 10.1007/bf00267236

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN: 0026-8925


  21 in total

1.  A restriction fragment analysis of the T7 left-early region.

Authors:  R A Ludwig; W C Summers
Journal:  Virology       Date:  1975-12       Impact factor: 3.616

2.  A restriction endonuclease from Staphylococcus aureus.

Authors:  J S Sussenbach; C H Monfoort; R Schiphof; E E Stobberingh
Journal:  Nucleic Acids Res       Date:  1976-11       Impact factor: 16.971

3.  pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long.

Authors:  J G Sutcliffe
Journal:  Nucleic Acids Res       Date:  1978-08       Impact factor: 16.971

4.  The terminal redundant regions of bacteriophage T7 DNA: their necessity for phage production studied by the infectivity of T7 DNA after modification by various exonucleases.

Authors:  B Dreiseikelmann; W Wackernagel
Journal:  Mol Gen Genet       Date:  1978-02-27

5.  Origin of concatemeric T7 DNA.

Authors:  J D Watson
Journal:  Nat New Biol       Date:  1972-10-18

6.  A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.

Authors:  I M Glynn; J B Chappell
Journal:  Biochem J       Date:  1964-01       Impact factor: 3.857

Review 7.  Bacteriophage T7 genetics.

Authors:  R Hausmann
Journal:  Curr Top Microbiol Immunol       Date:  1976       Impact factor: 4.291

8.  Transfection of restrictionless Escherichia coli by bacteriophage T7 dna: effect of in vitro erosion of DNA by gamma exonuclease.

Authors: 
Journal:  J Mol Biol       Date:  1976-08-25       Impact factor: 5.469

9.  The structure and maturation of intermediates in bacteriophage T7 DNA replication.

Authors:  L Langman; V Paetkau; D Scraba; R C Miller; G S Roeder; P D Sadowski
Journal:  Can J Biochem       Date:  1978-06

10.  In vivo effects of recBC DNase, exonuclease I, and DNA polymerases of Escherichia coli on the infectivity of native and single-stranded DNA of bacteriophage T7.

Authors:  K Seroka; W Wackernagel
Journal:  J Virol       Date:  1977-03       Impact factor: 5.103

View more
  1 in total

Review 1.  Bacteriophage T3 and bacteriophage T7 virus-host cell interactions.

Authors:  D H Krüger; C Schroeder
Journal:  Microbiol Rev       Date:  1981-03
  1 in total

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