| Literature DB >> 6928693 |
D Katcoff, U Nudel, D Zevin-Sonkin, Y Carmon, M Shani, H Lehrach, A M Frischauf, D Yaffe.
Abstract
The construction and partial characterization of recombinant bacterial plasmids carrying DNA sequences that hybridize with rat skeletal muscle actin and a myosin light chain mRNA is described. DNA of one clone hybridizes specifically with the muscle-specific alpha-actin mRNA. Three plasmid clones contain DNA inserts that hybridize with muscle as well as with nonmuscle actin mRNA. A fifth plasmid contains sequences complementary to mRNA coding for myosin light chain 2. DNA of this plasmid hybridizes specifically with RNA extracted from muscle and differentiated muscle cultures but not with RNA extracted from proliferating mononucleated myogenic cells.Entities:
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Year: 1980 PMID: 6928693 PMCID: PMC348403 DOI: 10.1073/pnas.77.2.960
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN: 0027-8424 Impact factor: 11.205