| Literature DB >> 3858807 |
U Nudel, D Greenberg, C P Ordahl, O Saxel, S Neuman, D Yaffe.
Abstract
To test the evolutionary conservation of DNA sequences specifying the developmentally regulated expression of the skeletal muscle actin gene, a recombinant plasmid containing the chicken skeletal muscle actin gene was introduced into rat myogenic cells. In a significant number of isolated clones, the accumulation of chicken actin mRNA increased greatly during differentiation. To test the expression in myogenic cells of a gene that is normally expressed during terminal differentiation of another tissue, rat myogenic cells were transfected with a mouse/human beta-globin chimeric gene. A decrease by a factor of 2-3 in the amount of globin mRNA during differentiation was observed in most clones in which the gene was expressed. The results indicate the conservation of the muscle-specific regulatory DNA sequences for more than 300 Myr.Entities:
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Year: 1985 PMID： 3858807 PMCID： PMC397723 DOI： 10.1073/pnas.82.10.3106
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205