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Literature DB >> 6894330

Skeletal muscle actin mRNA. Characterization of the 3' untranslated region.

M Shani, U Nudel, D Zevin-Sonkin, R Zakut, D Givol, D Katcoff, Y Carmon, J Reiter, A M Frischauf, D Yaffe.

Abstract

Plasmids p749, p106, and p150 contain cDNA inserts complementary to rat skeletal muscle actin mRNA. Nucleotide sequence analysis indicates the following sequence relationships: p749 specifies codons 171 to 360; p150 specifies codons 357 to 374 together with 120 nucleotides of the 3'-non-translated region; p106 specifies the last actin amino acid codon, the termination codon and the entire 3' non-translated region. Plasmid p749 hybridized with RNA extracted from rat skeletal muscle, cardiac muscle, smooth (stomach) muscle, and from brain. It also hybridizes well with RNA extracted from skeletal muscle and brain of dog and chick. Plasmid p106 hybridized specifically with rat striated muscles (skeletal and cardiac muscle) mRNA but not with mRNA from rat stomach and from rat brain. It also hybridized to RNA extracted from skeletal muscle of rabbit and dog but not from chick. Thermal stability of the hybrids and sensitivity to S1 digestion also indicated substantial divergence between the 3' untranslated end of rat and dog skeletal muscle actins. The investigation shows that the coding regions of actin genes are highly conserved, whereas the 3' non-coding regions diverged considerably during evolution. Probes constructed from the 3' non-coding regions of actin mRNAs can be used to identify the various actin mRNA and actin genes.

Entities: Gene Species

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Year: 1981 PMID： 6894330 PMCID： PMC327223 DOI： 10.1093/nar/9.3.579

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

30 in total

10. The development expression of the rat alpha-vascular and gamma-enteric smooth muscle isoactins: isolation and characterization of a rat gamma-enteric actin cDNA.

Authors: K M McHugh; J L Lessard
Journal: Mol Cell Biol Date: 1988-12 Impact factor: 4.272

Skeletal muscle actin mRNA. Characterization of the 3' untranslated region.

1. Non-identity of muscle and non-muscle actins.

2. Identification and characterization of multiple forms of actin.

3. Chick cytoplasmic actin and muscle actin have different structural genes.

4. Protein synthesis and actin heterogeneity in calf muscle cells in culture.

5. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.

6. 3' non-coding region sequences in eukaryotic messenger RNA.

7. The primary structure of actin from rabbit skeletal muscle. Completion and analysis of the amino acid sequence.

8. Human heart and platelet actins are products of different genes.

9. Actin microheterogeneity in chick embryo fibroblasts.

10. Nucleotide sequence of the rightward operator of phage lambda.

1. Inhibitory effects of interferon on the expression of genes regulated by platelet-derived growth factor.

2. A human beta-actin expression vector system directs high-level accumulation of antisense transcripts.

3. Cloning of alpha 2 chain of type VI collagen and expression during mouse development.

4. Expression of human alpha-tubulin genes: interspecies conservation of 3' untranslated regions.

5. There are approximately 20 actin gene in the human genome.

6. The nucleotide sequence of myosin light chain (L-2A) mRNA from embryonic chicken cardiac muscle tissue.

7. cDNA recombinant plasmid complementary to mRNAs for light chains 1 and 3 of mouse skeletal muscle myosin.

8. DNAase I sensitivity of genes expressed during myogenesis.

9. Expression and regulation of chicken actin genes introduced into mouse myogenic and nonmyogenic cells.

10. The development expression of the rat alpha-vascular and gamma-enteric smooth muscle isoactins: isolation and characterization of a rat gamma-enteric actin cDNA.