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Literature DB >> 6896998

Fusion of dipalmitoylphosphatidylcholine vesicles at 4 degrees C.

M Wong, F H Anthony, T W Tillack, T E Thompson.

Abstract

Small sonicated dipalmitoylphosphatidylcholine vesicles when incubated at 4 degrees C and high concentrations are shown to fuse completely to vesicles about 700-A diameter in 7 days, and these further fuse to about 950 A diameter vesicles after 3-4 weeks. The 950 A diameter vesicles are spherical, homogeneous, mostly unilamellar, have an internal aqueous space about 10 times that of small vesicles, and are stable for at least 6 months. The 950-A vesicles are characterized by agarose gel chromatography, freeze-fracturing electron microscopy, trapped volume measurements, differential scanning calorimetry, and diphenylhexatriene fluorescence polarization.

Entities: Chemical Gene

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Year: 1982 PMID： 6896998 DOI： 10.1021/bi00260a032

Source DB: PubMed Journal: Biochemistry ISSN： 0006-2960 Impact factor: 3.162

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Cited

10 in total

1. Membrane structural domains. Resolution limits using diphenylhexatriene fluorescence decay.

Authors: D A Barrow; B R Lentz
Journal: Biophys J Date: 1985-08 Impact factor: 4.033

2. Limited protection by small unilamellar liposomes against the renal tubular toxicity induced by repeated amphotericin B infusions in rats.

Authors: P Longuet; V Joly; P Amirault; N Seta; C Carbon; P Yeni
Journal: Antimicrob Agents Chemother Date: 1991-07 Impact factor: 5.191

Fusion of dipalmitoylphosphatidylcholine vesicles at 4 degrees C.

1. Membrane structural domains. Resolution limits using diphenylhexatriene fluorescence decay.

2. Limited protection by small unilamellar liposomes against the renal tubular toxicity induced by repeated amphotericin B infusions in rats.

3. Effect of surface curvature on the rate of cholesterol transfer between lipid vesicles.

4. Thermodynamic characterization of the pretransition of unilamellar dipalmitoyl-phosphatidylcholine vesicles.

5. Interactions between human defensins and lipid bilayers: evidence for formation of multimeric pores.

6. Highly Stable, Ultrasmall Polymer-Grafted Nanobins (usPGNs) with Stimuli-Responsive Capability.

7. Miscibility of phosphatidylcholine binary mixtures in unilamellar vesicles: phase equilibria.

8. Dose-dependent nonlinear response of the main phase-transition temperature of phospholipid membranes to alcohols.

9. Cholesterol transfer between lipid vesicles. Effect of phospholipids and gangliosides.

10. CD spectroscopy of peptides and proteins bound to large unilamellar vesicles.