Literature DB >> 6894453

Evidence for an altered structure of actin-S1 complexes when Mg-adenylylimidodiphosphate binds.

S B Marston.   

Abstract

Simultaneous measurements of actin-S1 binding and light scatter (or turbidity) were made in the presence and absence of saturating Mg-adenylylimidodiphosphate (Mg.AMP.PNP). It was found that if the actin was covalently labelled on cysteine 363 by dansyl aziridine (DAZ) the ternary complex DAZactin-S1-Mg.AMP.PNP scattered 36% less light than the binary complex DAZactin-S1. If the actin was not labelled there was no difference in scatter. The decrease in scatter of DAZactin-S1 was dependent on Mg.AMP.PNP concentration in a hyperbolic manner with half maximal decrease at 40 micrometers. No change in light scatter by DAZactin-S1 was found when H.AMP.PNP or Mg.ADP bound. The change in light scatter is evidence that the structure of DAZactin-S1 is altered when Mg.AMP.PNP binds. The possible nature of the structural change and its role in chemo-mechanical transduction is discussed.

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Year:  1980        PMID： 6894453     DOI： 10.1007/bf00711933

Source DB:  PubMed          Journal:  J Muscle Res Cell Motil        ISSN： 0142-4319            Impact factor:   2.698


  30 in total

1.  Energetics and mechanism of actomyosin adenosine triphosphatase.

Authors:  H D White; E W Taylor
Journal:  Biochemistry       Date:  1976-12-28       Impact factor: 3.162

2.  Low-angle x-ray diagrams from skeletal muscle: the effect of AMP-PNP, a non-hydrolyzed analogue of ATP.

Authors:  R W Lymn
Journal:  J Mol Biol       Date:  1975-12-25       Impact factor: 5.469

3.  Changes in muscle crossbridges when beta, gamma-imido-ATP binds to myosin.

Authors:  S B Marston; C D Rodger; R T Tregear
Journal:  J Mol Biol       Date:  1976-06-14       Impact factor: 5.469

4.  The structural basis of muscular contraction.

Authors:  H E Huxley
Journal:  Proc R Soc Lond B Biol Sci       Date:  1971-06-29

5.  Dynamics of F-actin and F-actin complexes.

Authors:  F D Carlson; A B Fraser
Journal:  J Mol Biol       Date:  1974-10-25       Impact factor: 5.469

6.  Studies on the kinetics of formation and dissociation of the actomyosin complex.

Authors:  B Finlayson; R W Lymn; E W Taylor
Journal:  Biochemistry       Date:  1969-03       Impact factor: 3.162

7.  Mechanism of adenosine triphosphate hydrolysis by actomyosin.

Authors:  R W Lymn; E W Taylor
Journal:  Biochemistry       Date:  1971-12-07       Impact factor: 3.162

8.  Characterization of sulfhydryl groups of actin.

Authors:  C J Lusty; H Fasold
Journal:  Biochemistry       Date:  1969-07       Impact factor: 3.162

9.  Studies on the isolation and molecular properties of homogeneous globular actin. Evidence for a single polypeptide chain structure.

Authors:  M K Rees; M Young
Journal:  J Biol Chem       Date:  1967-10-10       Impact factor: 5.157

10.  Myosin ATP hydrolysis: a mechanism involving a magnesium chelate complex.

Authors:  M Burke; E Reisler; W F Harrington
Journal:  Proc Natl Acad Sci U S A       Date:  1973-12       Impact factor: 11.205
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  6 in total

Review 1.  The dynamics of actin and myosin association and the crossbridge model of muscle contraction.

Authors:  M A Geeves
Journal:  Biochem J       Date:  1991-02-15       Impact factor: 3.857

2.  The inhibition of muscle contraction by adenosine 5' (beta, gamma-imido) triphosphate and by pyrophosphate.

Authors:  E Pate; R Cooke
Journal:  Biophys J       Date:  1985-06       Impact factor: 4.033

3.  The use of actin labelled with N-(1-pyrenyl)iodoacetamide to study the interaction of actin with myosin subfragments and troponin/tropomyosin.

Authors:  A H Criddle; M A Geeves; T Jeffries
Journal:  Biochem J       Date:  1985-12-01       Impact factor: 3.857

4.  The rates of formation and dissociation of actin-myosin complexes. Effects of solvent, temperature, nucleotide binding and head-head interactions.

Authors:  S B Marston
Journal:  Biochem J       Date:  1982-05-01       Impact factor: 3.857

5.  Modification of crossbridge states by ethylene glycol in insect flight muscle.

Authors:  M L Clarke; C D Rodger; R T Tregear
Journal:  J Muscle Res Cell Motil       Date:  1984-02       Impact factor: 2.698

6.  Modification of the interactions of myosin with actin and 5'-adenylyl imidodiphosphate by substitution of ethylene glycol for water.

Authors:  S B Marston; R T Tregear
Journal:  Biochem J       Date:  1984-01-01       Impact factor: 3.857
  6 in total

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