Literature DB >> 3911945

The use of actin labelled with N-(1-pyrenyl)iodoacetamide to study the interaction of actin with myosin subfragments and troponin/tropomyosin.

A H Criddle, M A Geeves, T Jeffries.   

Abstract

A pyrene label attached to Cys-374 of actin has been shown to be a useful probe for monitoring the interaction of actin with myosin subfragments [Kouyama & Mihashi (1981) Eur. J. Biochem. 114, 33-38]. We report that the presence of this label decreases the affinity of actin for myosin subfragment 1 by less than a factor of 2. The rate of actin binding is unaffected by the label and the dissociation rate is increased by up to a factor of 2. Both the rate of actin binding to, and the rate of actin dissociation from, heavy meromyosin show two phases when monitored by pyrene fluorescence. Thin filiments reconstituted from pyrene-labelled actin show a 5% increase in pyrene fluorescence on binding Ca2+.

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Year:  1985        PMID： 3911945      PMCID： PMC1152885          DOI： 10.1042/bj2320343

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  28 in total

1.  Separation of subfragment-1 isoenzymes from rabbit skeletal muscle myosin.

Authors:  A G Weeds; R S Taylor
Journal:  Nature       Date:  1975-09-04       Impact factor: 49.962

2.  The dissociation constant of the actin-heavy meromyosin subfragment-1 complex.

Authors:  S Marston; A Weber
Journal:  Biochemistry       Date:  1975-08-26       Impact factor: 3.162

3.  Energetics and mechanism of actomyosin adenosine triphosphatase.

Authors:  H D White; E W Taylor
Journal:  Biochemistry       Date:  1976-12-28       Impact factor: 3.162

4.  Affinity of myosin S-1 for F-actin, measured by time-resolved fluorescence anisotropy.

Authors:  S Highsmith; R A Mendelson; M F Morales
Journal:  Proc Natl Acad Sci U S A       Date:  1976-01       Impact factor: 11.205

5.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

6.  Studies on the kinetics of formation and dissociation of the actomyosin complex.

Authors:  B Finlayson; R W Lymn; E W Taylor
Journal:  Biochemistry       Date:  1969-03       Impact factor: 3.162

7.  Pressure-relaxation studies of pyrene-labelled actin and myosin subfragment 1 from rabbit skeletal muscle. Evidence for two states of acto-subfragment 1.

Authors:  J H Coates; A H Criddle; M A Geeves
Journal:  Biochem J       Date:  1985-12-01       Impact factor: 3.857

8.  Intrinsic fluorescence of actin.

Authors:  S S Lehrer; G Kerwar
Journal:  Biochemistry       Date:  1972-03-28       Impact factor: 3.162

9.  Kinetic properties of binding of myosin subfragment-1 with F-actin in the absence of nucleotide.

Authors:  M Yasui; T Arata; A Inoue
Journal:  J Biochem       Date:  1984-12       Impact factor: 3.387

10.  The magnesium-ion-dependent adenosine triphosphatase of bovine cardiac Myosin and its subfragment-1.

Authors:  R S Taylor; A G Weeds
Journal:  Biochem J       Date:  1976-11       Impact factor: 3.857
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  94 in total

1.  A flash photolysis fluorescence/light scattering apparatus for use with sub microgram quantities of muscle proteins.

Authors:  S Weiss; I Chizhov; M A Geeves
Journal:  J Muscle Res Cell Motil       Date:  2000       Impact factor: 2.698

2.  Three-dimensional reconstruction of thin filaments containing mutant tropomyosin.

Authors:  M Rosol; W Lehman; R Craig; C Landis; C Butters; L S Tobacman
Journal:  Biophys J       Date:  2000-02       Impact factor: 4.033

3.  Kinetic studies on the effects of ADP and ionic strength on the interaction between myosin subfragment-1 and actin: implications for load-sensitivity and regulation of the crossbridge cycle.

Authors:  P B Conibear
Journal:  J Muscle Res Cell Motil       Date:  1999-11       Impact factor: 2.698

4.  Interaction of myosin with F-actin: time-dependent changes at the interface are not slow.

Authors:  J Van Dijk; F Céline; T Barman; P Chaussepied
Journal:  Biophys J       Date:  2000-06       Impact factor: 4.033

5.  Toxoplasma gondii myosin A and its light chain: a fast, single-headed, plus-end-directed motor.

Authors:  Angelika Herm-Götz; Stefan Weiss; Rolf Stratmann; Setsuko Fujita-Becker; Christine Ruff; Edgar Meyhöfer; Thierry Soldati; Dietmar J Manstein; Michael A Geeves; Dominique Soldati
Journal:  EMBO J       Date:  2002-05-01       Impact factor: 11.598

6.  Mutations in the relay loop region result in dominant-negative inhibition of myosin II function in Dictyostelium.

Authors:  Georgios Tsiavaliaris; Setsuko Fujita-Becker; Renu Batra; Dmitrii I Levitsky; F Jon Kull; Michael A Geeves; Dietmar J Manstein
Journal:  EMBO Rep       Date:  2002-10-22       Impact factor: 8.807

7.  Interactions of the two heads of scallop (Argopecten irradians) heavy meromyosin with actin: influence of calcium and nucleotides.

Authors:  Miklos Nyitrai; Andrew G Szent-Györgyi; Michael A Geeves
Journal:  Biochem J       Date:  2003-03-15       Impact factor: 3.857

8.  Modification of interface between regulatory and essential light chains hampers phosphorylation-dependent activation of smooth muscle myosin.

Authors:  Shaowei Ni; Feng Hong; Brian D Haldeman; Josh E Baker; Kevin C Facemyer; Christine R Cremo
Journal:  J Biol Chem       Date:  2012-05-01       Impact factor: 5.157

9.  Cardiac myosin binding protein C and its phosphorylation regulate multiple steps in the cross-bridge cycle of muscle contraction.

Authors:  Arthur T Coulton; Julian E Stelzer
Journal:  Biochemistry       Date:  2012-04-06       Impact factor: 3.162

10.  The effect of toxins on inorganic phosphate release during actin polymerization.

Authors:  Andrea Vig; Róbert Ohmacht; Eva Jámbor; Beáta Bugyi; Miklós Nyitrai; Gábor Hild
Journal:  Eur Biophys J       Date:  2011-01-04       Impact factor: 1.733
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