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Literature DB >> 6893626

Brain tubulin and actin cDNA sequences: isolation of recombinant plasmids.

I Ginzburg, A de Baetselier, M D Walker, L Behar, H Lehrach, A M Frischauf, U Z Littauer.

Abstract

Rat brain mRNA enriched for tubulin and actin sequences was used to prepare double stranded cDNA. A library of recombinant clones was constructed by inserting the dsDNA into the Pst1 site of pBR322 plasmid and transformation of E. coli chi 1776 host. Clones bearing sequences coding for tubulin and actin were identified and characterized.

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Year: 1980 PMID： 6893626 PMCID： PMC324174 DOI： 10.1093/nar/8.16.3553

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

23 in total

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