Literature DB >> 687376

A spectrophotometric method for the detection of contaminant chymopapains in preparations of papain. Selective modification of one type of thiol group in the chymopapains by a two-protonic-state reagent.

B S Baines, K Brocklehurst.   

Abstract

A simple spectrophotometric method for the detection of chymopapains A and B, contaminants in some preparations of papain (EC 3.422.2), was devised. If the amount of rapidly reacting thiol in the preparation, as determined by increase in A343 consequent on reaction with the two-protonic-state thiol titrant, 2,2' -dipyridl disulphide, is greater at PH8-9 than at pH4, contamination by the chymopapains is in dicated.

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Year:  1978        PMID： 687376      PMCID： PMC1185781          DOI： 10.1042/bj1730345

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  12 in total

1.  Chymopapain. I. Isolation, crystallization, and preliminary characterization.

Authors:  M EBATA; K T YASUNOBU
Journal:  J Biol Chem       Date:  1962-04       Impact factor: 5.157

2.  Crystalline papain. I. Preparation, specificity, and activation.

Authors:  J R KIMMEL; E L SMITH
Journal:  J Biol Chem       Date:  1954-04       Impact factor: 5.157

3.  Isolation and characterization of papaya peptidase A from commercial chymopapain.

Authors:  G W Robinson
Journal:  Biochemistry       Date:  1975-08-12       Impact factor: 3.162

4.  A reporter group delivery system with both absolute and selective specificity for thiol groups and an improved fluorescent probe containing the 7-nitrobenzo-2-oxa-1,3-diazole moiety.

Authors:  T Stuchbury; M Shipton; R Norris; J P Malthouse; K Brocklehurst; J A Herbert; H Suschitzky
Journal:  Biochem J       Date:  1975-11       Impact factor: 3.857

5.  Characterization of the papain active centre by using two-protonic-state electrophiles as reactivity probes. Evidence for nucleophilic reactivity in the un-interrupted cysteine-25-histidine-159 interactive system.

Authors:  M Shipton; K Brochlehurst
Journal:  Biochem J       Date:  1978-05-01       Impact factor: 3.857

6.  Covalent chromatography by thiol-disulfide interchange.

Authors:  K Brocklehurst; J Carlsson; M P Kierstan; E M Crook
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

7.  Covalent chromatography. Preparation of fully active papain from dried papaya latex.

Authors:  K Brocklehurst; J Carlsson; M P Kierstan; E M Crook
Journal:  Biochem J       Date:  1973-07       Impact factor: 3.857

8.  The activation reaction of papain.

Authors:  L A Sluyterman
Journal:  Biochim Biophys Acta       Date:  1967-07-11

9.  Reactions of papain and of low-molecular-weight thiols with some aromatic disulphides. 2,2'-Dipyridyl disulphide as a convenient active-site titrant for papain even in the presence of other thiols.

Authors:  K Brocklehurst; G Little
Journal:  Biochem J       Date:  1973-05       Impact factor: 3.857

10.  Preparation and characterization of enzymes from spray-dried papaya (Carica papaya) latex [proceedings].

Authors:  B S Baines; T Stuchbury; K Brocklehurst
Journal:  Biochem Soc Trans       Date:  1978       Impact factor: 5.407
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  19 in total

1.  Evaluation of hydrogen-bonding and enantiomeric P2-S2 hydrophobic contacts in dynamic aspects of molecular recognition by papain.

Authors:  M Patel; I S Kayani; W Templeton; G W Mellor; E W Thomas; K Brocklehurst
Journal:  Biochem J       Date:  1992-11-01       Impact factor: 3.857

2.  Temperature-dependences of the kinetics of reactions of papain and actinidin with a series of reactivity probes differing in key molecular recognition features.

Authors:  Sheraz Gul; Geoffrey W Mellor; Emrys W Thomas; Keith Brocklehurst
Journal:  Biochem J       Date:  2006-05-15       Impact factor: 3.857

3.  Variation in aspects of cysteine proteinase catalytic mechanism deduced by spectroscopic observation of dithioester intermediates, kinetic analysis and molecular dynamics simulations.

Authors:  J D Reid; S Hussain; S K Sreedharan; T S Bailey; S Pinitglang; E W Thomas; C S Verma; K Brocklehurst
Journal:  Biochem J       Date:  2001-07-15       Impact factor: 3.857

4.  A thiol-labelling reagent and reactivity probe containing electrophilic mercury and a chromophoric leaving group.

Authors:  B S Baines; K Brocklehurst
Journal:  Biochem J       Date:  1979-06-01       Impact factor: 3.857

5.  Identification of signalling and non-signalling binding contributions to enzyme reactivity. Alternative combinations of binding interactions provide for change in transition-state geometry in reactions of papain.

Authors:  D Kowlessur; C M Topham; E W Thomas; M O'Driscoll; W Templeton; K Brocklehurst
Journal:  Biochem J       Date:  1989-03-15       Impact factor: 3.857

6.  Clarification of the pH-dependent kinetic behaviour of papain by using reactivity probes and analysis of alkylation and catalysed acylation reactions in terms of multihydronic state models: implications for electrostatics calculations and interpretation of the consequences of site-specific mutations such as Asp-158-Asn and Asp-158-Glu.

Authors:  G W Mellor; M Patel; E W Thomas; K Brocklehurst
Journal:  Biochem J       Date:  1993-08-15       Impact factor: 3.857

7.  Variation in the pH-dependent pre-steady-state and steady-state kinetic characteristics of cysteine-proteinase mechanism: evidence for electrostatic modulation of catalytic-site function by the neighbouring carboxylate anion.

Authors:  Syeed Hussain; Surapong Pinitglang; Tamara S F Bailey; James D Reid; Michael A Noble; Marina Resmini; Emrys W Thomas; Richard B Greaves; Chandra S Verma; Keith Brocklehurst
Journal:  Biochem J       Date:  2003-06-15       Impact factor: 3.857

8.  Benzyloxycarbonylphenylalanylcitrulline p-nitroanilide as a substrate for papain and other plant cysteine proteinases.

Authors:  C J Gray; J Boukouvalas; R J Szawelski; C W Wharton
Journal:  Biochem J       Date:  1984-04-01       Impact factor: 3.857

9.  Ionization characteristics of the Cys-25/His-159 interactive system and of the modulatory group of papain: resolution of ambiguity by electronic perturbation of the quasi-2-mercaptopyridine leaving group in a new pyrimidyl disulphide reactivity probe.

Authors:  G W Mellor; E W Thomas; C M Topham; K Brocklehurst
Journal:  Biochem J       Date:  1993-02-15       Impact factor: 3.857

10.  Evidence that the active centre of chymopapain A is different from the active centres of some other cysteine proteinases and that the Brønsted coefficient (beta nuc.) for the reactions of thiolate anions with 2,2'-dipyridyl disulphide may be decreased by reagent protonation.

Authors:  K Brocklehurst; B S Baines; M S Mushiri
Journal:  Biochem J       Date:  1980-07-01       Impact factor: 3.857
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