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Literature DB >> 38780

A thiol-labelling reagent and reactivity probe containing electrophilic mercury and a chromophoric leaving group.

Abstract

2-(2'-Pyridylmercapto)mercuri-4-nitrophenol was synthesized and evaluated as a thiol-labelling reagent containing a chromophoric leaving group and as a reactivity probe by studies on its reactions with 2-mercaptoethanol and with papain (EC 3.4.22.2).

Entities: Chemical

Mesh：

Substances：

Year: 1979 PMID： 38780 PMCID： PMC1186680 DOI： 10.1042/bj1790701

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

11 in total

1. USE OF "REPORTER GROUPS" IN STRUCTURE-FUNCTION STUDIES OF PROTEINS.

Authors: M BURR; D E KOSHLAND
Journal: Proc Natl Acad Sci U S A Date: 1964-10 Impact factor: 11.205

2. Spectrochemical and ligand-binding studies of an active mercurinitrophenol-labeled creatine kinase.

Authors: M C Laue; F A Quiocho
Journal: Biochemistry Date: 1977-08-23 Impact factor: 3.162

3. A reporter group delivery system with both absolute and selective specificity for thiol groups and an improved fluorescent probe containing the 7-nitrobenzo-2-oxa-1,3-diazole moiety.

Authors: T Stuchbury; M Shipton; R Norris; J P Malthouse; K Brocklehurst; J A Herbert; H Suschitzky
Journal: Biochem J Date: 1975-11 Impact factor: 3.857

4. The pH-dependence of second-order rate constants of enzyme modification may provide free-reactant pKa values.

Authors: K Brocklehurst; H B Dixon
Journal: Biochem J Date: 1977-12-01 Impact factor: 3.857

5. The reaction of creatine kinase with 2-chloromercuri-4-nitrophenol.

Authors: F A Quiocho; J S Olson
Journal: J Biol Chem Date: 1974-09-25 Impact factor: 5.157

Review 6. Stoicheiometric, reactivity and environmental probes for sulphydryl groups in enzymes.

Authors: H Gutfreund; C H McMurray
Journal: Biochem Soc Symp Date: 1970

7. Preparation of fully active ficin from Ficus glabrata by covalent chromatography and characterization of its active centre by using 2,2'-depyridyl disulphide as a reactivity probe.

Authors: J P Malthouse; K Brocklehurst
Journal: Biochem J Date: 1976-11 Impact factor: 3.857

8. Reactions of L-ergothioneine and some other aminothiones with2,2'-and 4,4'-dipyridyl disulphides and of L-ergothioneine with iodoacetamide. 2-Mercaptoimidazoles, 2- and 4-thiopyridones, thiourea and thioacetamide as highly reactive neutral sulphur nucleophils.

Authors: J Carlsson; M P Kierstan; K Brocklehurst
Journal: Biochem J Date: 1974-04 Impact factor: 3.857