Literature DB >> 6846834

Quantitative binding of covalently closed circular DNA to nitrocellulose in NaI.

J Bresser, D Gillespie.   

Abstract

A procedure for quantitatively binding covalently closed circular DNA to nitrocellulose is detailed. The procedure involves making DNA saturated with respect to Nat at 25 degrees C, denaturing the DNA by heating to 80-100 degrees C, then passing the DNA-NaI solution through a nitrocellulose membrane at 50-60 degrees C. Form I DNA remains denatured throughout the loading procedure and is quantitatively deposited on the membrane as denatured DNA. The mechanism of binding DNA to nitrocellulose in NaI appears to be different from the binding promoted by NaCl.

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Year:  1983        PMID： 6846834     DOI： 10.1016/0003-2697(83)90562-6

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  5 in total

1.  Bioreactor sensors based on nucleic acid hybridization reactions. Scientific note.

Authors:  R C Moore; J W Blackburn; P R Bienkowski; G S Sayler
Journal:  Appl Biochem Biotechnol       Date:  1988-04       Impact factor: 2.926

2.  A species-specific DNA probe for the detection of Mycoplasma gallisepticum.

Authors:  M Santha; K Burg; I Rasko; L Stipkovits
Journal:  Infect Immun       Date:  1987-11       Impact factor: 3.441

3.  Duplication of Escherichia coli during inhibition of net phospholipid synthesis.

Authors:  O Pierucci; M Rickert
Journal:  J Bacteriol       Date:  1985-04       Impact factor: 3.490

4.  Biological activity of mRNA immobilized on nitrocellulose in NaI.

Authors:  J Bresser; H R Hubbell; D Gillespie
Journal:  Proc Natl Acad Sci U S A       Date:  1983-11       Impact factor: 11.205

5.  Immobilization of polynucleotides on magnetic particles. Factors influencing hybridization efficiency.

Authors:  P J Day; P S Flora; J E Fox; M R Walker
Journal:  Biochem J       Date:  1991-09-15       Impact factor: 3.857
  5 in total

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