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Literature DB >> 3666965

A species-specific DNA probe for the detection of Mycoplasma gallisepticum.

M Santha¹, K Burg, I Rasko, L Stipkovits.

Abstract

An 800-base-pair DNA fragment from a partial genomic library of Mycoplasma gallisepticum was selected and used as a probe for the selective detection of this avian pathogen. The specificity and sensitivity of this probe were demonstrated by using dot blot and Southern hybridizations.

Mesh：

Substances：
DNA, Bacterial

Year: 1987 PMID： 3666965 PMCID： PMC259991 DOI： 10.1128/iai.55.11.2857-2859.1987

Source DB: PubMed Journal: Infect Immun ISSN： 0019-9567 Impact factor: 3.441

14 in total

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

2. Elution of DNA from agarose gels after electrophoresis.

Authors: R Yang; J Lis; R Wu
Journal: Methods Enzymol Date: 1979 Impact factor: 1.600

3. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors: H C Birnboim; J Doly
Journal: Nucleic Acids Res Date: 1979-11-24 Impact factor: 16.971

4. Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid.

Authors: E M Lederberg; S N Cohen
Journal: J Bacteriol Date: 1974-09 Impact factor: 3.490

5. Bovine mycoplasmas: cultural and biochemical studies. I.

Authors: H Erno; L Stipkovits
Journal: Acta Vet Scand Date: 1973 Impact factor: 1.695

6. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

Authors: A P Feinberg; B Vogelstein
Journal: Anal Biochem Date: 1983-07-01 Impact factor: 3.365

Review 7. Molecular biology and genetics of mycoplasmas (Mollicutes).

Authors: S Razin
Journal: Microbiol Rev Date: 1985-12

8. Isolation of high-molecular-weight DNA from mammalian cells.

Authors: M Gross-Bellard; P Oudet; P Chambon
Journal: Eur J Biochem Date: 1973-07-02

9. Detection of mycoplasmas infecting cell cultures by DNA hybridization.

Authors: S Razin; M Gross; M Wormser; Y Pollack; G Glaser
Journal: In Vitro Date: 1984-05

10. Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots.

Authors: J J Leary; D J Brigati; D C Ward
Journal: Proc Natl Acad Sci U S A Date: 1983-07 Impact factor: 11.205

1 in total

1. Molecular detection and phylogenetic analysis of Mycoplasma gallisepticum from backyard and commercial turkey flocks in Iran.

Authors: Saeed Rasoulinezhad; Mohammad Hassan Bozorgmehrifard; Hossein Hosseini; Nariman Sheikhi; Saeed Charkhkar
Journal: Vet Res Forum Date: 2017-12-15 Impact factor: 1.054

1 in total