Literature DB >> 6579539

Biological activity of mRNA immobilized on nitrocellulose in NaI.

J Bresser, H R Hubbell, D Gillespie.   

Abstract

In 12.2 molal NaI and at 25 degrees C or below, mRNA bound to nitrocellulose while DNA and rRNA did not. Neither the poly(A) tract nor the cap were required for binding. The immobilized RNA could be translated, reverse transcribed, hybridized with radioactive probes, or released for further manipulation. mRNA was efficiently transferred from polyacrylamide to nitrocellulose in NaI. Baking was not required to fix NaI-immobilized mRNA to nitrocellulose. When cells dissolved in 12.2 molal NaI were filtered through nitrocellulose, mRNA became selectively bound (quickblot). The quick-blot system utilizing protease and detergents to prepare cells for NaI solubilization was especially suitable in quantitative, rapid screening of cells for expression of specific genes. Expression of highly repeated DNA sequences was detected in human leukemia cells.

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Year:  1983        PMID： 6579539      PMCID： PMC390385          DOI： 10.1073/pnas.80.21.6523

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  17 in total

1.  A method for the detection of RNA-DNA complexes.

Authors:  A P NYGAARD; B D HALL
Journal:  Biochem Biophys Res Commun       Date:  1963-07-18       Impact factor: 3.575

2.  Electrophoretic patterns of deadenylylated chorion and globin mRNAs.

Authors:  J N Vournakis; A Efstratiadis; F C Kafatos
Journal:  Proc Natl Acad Sci U S A       Date:  1975-08       Impact factor: 11.205

3.  Nucleic acid hybridization using DNA covalently coupled to cellulose.

Authors:  B E Noyes; G R Stark
Journal:  Cell       Date:  1975-07       Impact factor: 41.582

4.  A novel phosphodiesterase from cultured tobacco cells.

Authors:  H Shinshi; M Miwa; K Kato; M Noguchi; T Matsushima; T Sugimura
Journal:  Biochemistry       Date:  1976-05-18       Impact factor: 3.162

5.  The binding of RNA to various celluloses.

Authors:  J DeLarco; G Guroff
Journal:  Biochem Biophys Res Commun       Date:  1973-01-23       Impact factor: 3.575

6.  A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane.

Authors:  D Gillespie; S Spiegelman
Journal:  J Mol Biol       Date:  1965-07       Impact factor: 5.469

7.  Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

Authors:  P S Thomas
Journal:  Proc Natl Acad Sci U S A       Date:  1980-09       Impact factor: 11.205

8.  Enzymatic in vitro synthesis of globin genes.

Authors:  A Efstratiadis; F C Kafatos; A M Maxam; T Maniatis
Journal:  Cell       Date:  1976-02       Impact factor: 41.582

9.  Inhibition of intractable nucleases with ribonucleoside--vanadyl complexes: isolation of messenger ribonucleic acid from resting lymphocytes.

Authors:  S L Berger; C S Birkenmeier
Journal:  Biochemistry       Date:  1979-11-13       Impact factor: 3.162

10.  Structure and functions of the Kirsten murine sarcoma virus genome: molecular cloning of biologically active Kirsten murine sarcoma virus DNA.

Authors:  N Tsuchida; S Uesugi
Journal:  J Virol       Date:  1981-05       Impact factor: 5.103
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  9 in total

1.  Gene expression of pigment-binding proteins of the bacterial photosynthetic apparatus: Transcription and assembly in the membrane of Rhodopseudomonas capsulata.

Authors:  G Klug; N Kaufmann; G Drews
Journal:  Proc Natl Acad Sci U S A       Date:  1985-10       Impact factor: 11.205

2.  DNA-mediated transformation of Chlamydomonas reinhardi cells: use of aminoglycoside 3'-phosphotransferase as a selectable marker.

Authors:  S E Hasnain; E K Manavathu; W C Leung
Journal:  Mol Cell Biol       Date:  1985-12       Impact factor: 4.272

3.  Nucleotide sequence and expression of a phosphate-regulated gene encoding a secreted hemolysin of Pseudomonas aeruginosa.

Authors:  A E Pritchard; M L Vasil
Journal:  J Bacteriol       Date:  1986-07       Impact factor: 3.490

4.  Lineage-specific transformation after differentiation of multipotential murine stem cells containing a human oncogene.

Authors:  J C Bell; K Jardine; M W McBurney
Journal:  Mol Cell Biol       Date:  1986-02       Impact factor: 4.272

5.  Isolation of hybridomas expressing a specific heavy chain variable region gene segment by using a screening technique that detects mRNA sequences in whole cell lysates.

Authors:  T Manser; M L Gefter
Journal:  Proc Natl Acad Sci U S A       Date:  1984-04       Impact factor: 11.205

6.  Distinctive transforming genes in x-ray-transformed mammalian cells.

Authors:  C Borek; A Ong; H Mason
Journal:  Proc Natl Acad Sci U S A       Date:  1987-02       Impact factor: 11.205

7.  Isolation of a pathogenic clone of mouse mammary tumor virus.

Authors:  D W Morris; H D Bradshaw; H T Billy; R J Munn; R D Cardiff
Journal:  J Virol       Date:  1989-01       Impact factor: 5.103

8.  The phenotypic and molecular characterization of Nb2 lymphoma cells activated with IL-2 and human growth hormone.

Authors:  J H Cox; J R Lamb; V Bal; G W Butcher; J C Howard; M J Owen; J Ivanyi
Journal:  Immunology       Date:  1989-01       Impact factor: 7.397

9.  Human immunodeficiency virus induction of malignant transformation in human B lymphocytes.

Authors:  J Laurence; S M Astrin
Journal:  Proc Natl Acad Sci U S A       Date:  1991-09-01       Impact factor: 11.205
  9 in total

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