Literature DB >> 681338

Human hypoxanthine-guanine phosphoribosyltransferase. Steady state kinetics of the forward and reverse reactions.

A Giacomello, C Salerno.   

Abstract

A steady state kinetic study of the hypoxanthine-guanine phosphoribosyltransferase-catalyzed reaction in the forward and the reverse directions was carried out. The results obtained favor a sequential mechanism where the monomagnesium complexes of IMP and PPi bind to the enzyme in a rapid equilibrium random fashion while products must dissociate from the enzyme in ordered sequence, first the purine base and then the magnesium complex(es) of P-Rib-PP.

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Year:  1978        PMID： 681338

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  10 in total

1.  tRNA-guanine transglycosylase from E. coli: a ping-pong kinetic mechanism is consistent with nucleophilic catalysis.

Authors:  DeeAnne M Goodenough-Lashua; George A Garcia
Journal:  Bioorg Chem       Date:  2003-08       Impact factor: 5.275

2.  Hypoxanthine phosphoribosyltransferase from Trypanosoma cruzi as a target for structure-based inhibitor design: crystallization and inhibition studies with purine analogs.

Authors:  A E Eakin; A Guerra; P J Focia; J Torres-Martinez; S P Craig
Journal:  Antimicrob Agents Chemother       Date:  1997-08       Impact factor: 5.191

3.  Validation and steady-state analysis of a power-law model of purine metabolism in man.

Authors:  R Curto; E O Voit; A Sorribas; M Cascante
Journal:  Biochem J       Date:  1997-06-15       Impact factor: 3.857

4.  An enzymatic spectrophotometric assay for inosinic acid.

Authors:  C Salerno; A Giacomello
Journal:  Experientia       Date:  1981-03-15

5.  Apparent equilibrium constant of the hypoxanthine guanine phosphoribosyltransferase-catalyzed IMP-GMP exchange.

Authors:  C Salerno; A Giacomello
Journal:  Experientia       Date:  1982-10-15

6.  Ternary complex structure of human HGPRTase, PRPP, Mg2+, and the inhibitor HPP reveals the involvement of the flexible loop in substrate binding.

Authors:  G K Balendiran; J A Molina; Y Xu; J Torres-Martinez; R Stevens; P J Focia; A E Eakin; J C Sacchettini; S P Craig
Journal:  Protein Sci       Date:  1999-05       Impact factor: 6.725

7.  Genotype-phenotype correlations in Lesch-Nyhan disease: moving beyond the gene.

Authors:  Rong Fu; H A Jinnah
Journal:  J Biol Chem       Date:  2011-12-07       Impact factor: 5.157

8.  Tetrahydrobiopterin deficiency and dopamine loss in a genetic mouse model of Lesch-Nyhan disease.

Authors:  K Hyland; S Kasim; K Egami; L A Arnold; H A Jinnah
Journal:  J Inherit Metab Dis       Date:  2004       Impact factor: 4.982

9.  Identification of a single nucleotide change in a mutant gene for hypoxanthine-guanine phosphoribosyltransferase (HPRT Ann Arbor).

Authors:  S Fujimori; Y Hidaka; B L Davidson; T D Palella; W N Kelley
Journal:  Hum Genet       Date:  1988-05       Impact factor: 4.132

10.  A spectrophotometric method for the determination of 5-phosphoribosyl-1-pyrophosphate.

Authors:  C Salerno; A Giacomello; E Messina
Journal:  Experientia       Date:  1979-08-15
  10 in total

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