Literature DB >> 477861

A spectrophotometric method for the determination of 5-phosphoribosyl-1-pyrophosphate.

C Salerno, A Giacomello, E Messina.   

Abstract

A new, sensitive, specific and simple spectrophotometric method for the determination of 5-phosphoribosyl-l-pyrophosphate (PRPP) is presented. PRPP is reacted with excess hypoxanthine in the presence of hypoxanthine-guanine phosphoribosyltransferase. At the end of the reaction, PRPP concentration is measured from the extent of conversion of hypoxanthine to inositate. The concentration of the purine base is determined spectrophotometrically in the presence of xanthine oxidase.

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Year:  1979        PMID： 477861     DOI： 10.1007/bf01949914

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  9 in total

1.  Enzymatic synthesis and properties of 5-phosphoribosylpyrophosphate.

Authors:  A KORNBERG; I LIEBERMAN; E S SIMMS
Journal:  J Biol Chem       Date:  1955-07       Impact factor: 5.157

2.  Differential spectrophotometry of purine compounds by means of specific enzymes; determination of hydroxypurine compounds.

Authors:  H M KALCKAR
Journal:  J Biol Chem       Date:  1947-02       Impact factor: 5.157

3.  Evaluation of the role of 5-phosphoribosyl-alpha-1-pyrophosphate synthetase in congenital hyperuricemia and gout: a simple isotopic assay and an activity stain for the enzyme.

Authors:  M G Johnson; S Rosenweig; R L Switzer; M A Becker; J E Seegmiller
Journal:  Biochem Med       Date:  1974-07

4.  A comparative study of purine metabolism by human and pig erythrocytes in vitro.

Authors:  B M Dean; H A Simmonds; A Cadenhead
Journal:  Biochem Pharmacol       Date:  1973-12-15       Impact factor: 5.858

5.  Simpler method for the determination of 5-phosphoribosyl-1-pyrophosphate in red blood cells.

Authors:  O Sperling; G Eilam; S Persky-Brosh; A De Vries
Journal:  J Lab Clin Med       Date:  1972-06

6.  Determination of 5-phosphoribosyl 1-pyrophosphate in mouse liver.

Authors:  M Lalanne; J F Henderson
Journal:  Anal Biochem       Date:  1974-11       Impact factor: 3.365

7.  The detection and identification of intermediates of the pentose phosphate cycle and related compounds.

Authors:  T Wood
Journal:  J Chromatogr       Date:  1968-06-18

8.  Human hypoxanthine-guanine phosphoribosyltransferase. Steady state kinetics of the forward and reverse reactions.

Authors:  A Giacomello; C Salerno
Journal:  J Biol Chem       Date:  1978-09-10       Impact factor: 5.157

9.  An improved method for the determination of 5-phosphoribosyl 1-pyrophosphate.

Authors:  V Micheli; G Pompucci; R Marcolongo
Journal:  Clin Chim Acta       Date:  1975-12-01       Impact factor: 3.786
  9 in total
  1 in total

1.  Apparent equilibrium constant of the hypoxanthine guanine phosphoribosyltransferase-catalyzed IMP-GMP exchange.

Authors:  C Salerno; A Giacomello
Journal:  Experientia       Date:  1982-10-15
  1 in total

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