Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Identification of pilin pools in the membranes of Pseudomonas aeruginosa.

Literature DB >> 6813311

Identification of pilin pools in the membranes of Pseudomonas aeruginosa.

Abstract

The proteins of purified inner and outer membranes obtained from Pseudomonas aeruginosa strains PAK and PAK/2Pfs were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to nitrocellulose, and treated with antiserum raised against pure pili. Bound antipilus antibodies were visualized by reaction with 125I-labeled protein A from Staphylococcus aureus. The results showed that there are pools of pilin in both the inner and outer membranes of P. aeruginosa and that the pool size in the multipiliated strain is comparable with that of the wild-type strain.

Entities: Chemical Species

Mesh：

Substances：

Year: 1982 PMID： 6813311 PMCID： PMC221517 DOI： 10.1128/jb.152.2.687-691.1982

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

13 in total

1. Outer membranes of gram-negative bacteria. XIX. Isolation from Pseudomonas aeruginosa PAO1 and use in reconstitution and definition of the permeability barrier.

Authors: R E Hancock; H Nikaido
Journal: J Bacteriol Date: 1978-10 Impact factor: 3.490

2. Electrophoretic resolution of the "major outer membrane protein" of Escherichia coli K12 into four bands.

Authors: B Lugtenberg; J Meijers; R Peters; P van der Hoek; L van Alphen
Journal: FEBS Lett Date: 1975-10-15 Impact factor: 4.124

3. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors: H Towbin; T Staehelin; J Gordon
Journal: Proc Natl Acad Sci U S A Date: 1979-09 Impact factor: 11.205

Identification of pilin pools in the membranes of Pseudomonas aeruginosa.

1. Outer membranes of gram-negative bacteria. XIX. Isolation from Pseudomonas aeruginosa PAO1 and use in reconstitution and definition of the permeability barrier.

2. Electrophoretic resolution of the "major outer membrane protein" of Escherichia coli K12 into four bands.

3. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

4. Culture medium for enterobacteria.

5. Association of newly synthesized major f1 coat protein with infected host cell inner membrane.

6. Succinate dehydrogenase of Escherichia coli membrane vesicles. Activation and properties of the enzyme.

7. Location of an F-pilin pool in the inner membrane.

8. Composition and molecular weight of pili purified from Pseudomonas aeruginosa K.

9. Outer membrane of Pseudomonas aeruginosa: heat- 2-mercaptoethanol-modifiable proteins.

10. Biochemical studies on pili isolated from Pseudomonas aeruginosa strain PAO.

1. Assembly of mutant pilins in Pseudomonas aeruginosa: formation of pili composed of heterologous subunits.

Review 2. Type IV pilin proteins: versatile molecular modules.

3. Use of a pilin gene probe to study molecular epidemiology of Pseudomonas aeruginosa.

4. Modulation of gonococcal piliation by regulatable transcription of pilE.

5. Gonococcal pili. Primary structure and receptor binding domain.

6. Cloning and expression of the pilin gene of Pseudomonas aeruginosa PAK in Escherichia coli.

7. Expression of the Pseudomonas aeruginosa PAK pilin gene in Escherichia coli.

8. Antibody response of infected mice to outer membrane proteins of Pseudomonas aeruginosa.

9. Mapping of export signals of Pseudomonas aeruginosa pilin with alkaline phosphatase fusions.

10. Mapping of the antigenic determinants of Pseudomonas aeruginosa PAK polar pili.