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Literature DB >> 2867992

Expression of the Pseudomonas aeruginosa PAK pilin gene in Escherichia coli.

Abstract

Pseudomonas aeruginosa is a piliated opportunistic pathogen. We have recently reported the cloning of the structural gene for the pilus protein, pilin, from P. aeruginosa PAK (B. L. Pasloske, B. B. Finlay, and W. Paranchych, FEBS Lett. 183:408-412, 1985), and in this paper we present evidence that this chimera (pBP001) expresses P. aeruginosa PAK pilin in Escherichia coli independent of a vector promoter. The strength of the promoter for the PAK pilin gene was assayed, and the cellular location of the pilin protein within E. coli was examined. This protein was present mainly in the inner membrane fraction both with and without its six-amino-acid leader sequence, but it was not assembled into pili.

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Year: 1986 PMID： 2867992 PMCID： PMC214465 DOI： 10.1128/jb.165.2.625-630.1986

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

31 in total

Review 1. Regulatory sequences involved in the promotion and termination of RNA transcription.

Authors: M Rosenberg; D Court
Journal: Annu Rev Genet Date: 1979 Impact factor: 16.830

2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

3. Pilus genes of Neisseria gonorrheae: chromosomal organization and DNA sequence.

Authors: T F Meyer; E Billyard; R Haas; S Storzbach; M So
Journal: Proc Natl Acad Sci U S A Date: 1984-10 Impact factor: 11.205

4. The spontaneous insertion of proteins into and across membranes: the helical hairpin hypothesis.

Authors: D M Engelman; T A Steitz
Journal: Cell Date: 1981-02 Impact factor: 41.582

5. Nucleotide sequence of the gene encoding pilin of Bacteroides nodosus, the causal organism of ovine footrot.

Authors: T C Elleman; P A Hoyne
Journal: J Bacteriol Date: 1984-12 Impact factor: 3.490

6. Repetitive extragenic palindromic sequences: a major component of the bacterial genome.

Authors: M J Stern; G F Ames; N H Smith; E C Robinson; C F Higgins
Journal: Cell Date: 1984-07 Impact factor: 41.582

7. A comprehensive sequence analysis program for the IBM personal computer.

Authors: C Queen; L J Korn
Journal: Nucleic Acids Res Date: 1984-01-11 Impact factor: 16.971

8. Cytoplasmic membrane vesicles of Escherichia coli. A simple method for preparing the cytoplasmic and outer membranes.

Authors: I Yamato; Y Anraku; K Hirosawa
Journal: J Biochem Date: 1975-04 Impact factor: 3.387

9. Identification of pilin pools in the membranes of Pseudomonas aeruginosa.

Authors: T H Watts; E A Worobec; W Paranchych
Journal: J Bacteriol Date: 1982-11 Impact factor: 3.490

10. A function of Pseudomonas aeruginosa PAO polar pili: twitching motility.

Authors: D E Bradley
Journal: Can J Microbiol Date: 1980-02 Impact factor: 2.419

14 in total

1. Overproduction of an acetylxylan esterase from the extreme thermophile "Caldocellum saccharolyticum" in Escherichia coli.

Authors: E Lüthi; N B Jasmat; P L Bergquist
Journal: Appl Microbiol Biotechnol Date: 1990-11 Impact factor: 4.813

2. Type IV pilus retraction in pathogenic Neisseria is regulated by the PilC proteins.

Authors: Philippe C Morand; Emmanuelle Bille; Sandrine Morelle; Emmanuel Eugène; Jean-Luc Beretti; Matthew Wolfgang; Thomas F Meyer; Michael Koomey; Xavier Nassif
Journal: EMBO J Date: 2004-04-22 Impact factor: 11.598

3. Characterization of the genome of Pseudomonas aeruginosa bacteriophage phi PLS27 with particular reference to the ends of the DNA.

Authors: B J Allan; P Davies; E B Carstens; A M Kropinski
Journal: J Virol Date: 1989-04 Impact factor: 5.103

Expression of the Pseudomonas aeruginosa PAK pilin gene in Escherichia coli.

Review 1. Regulatory sequences involved in the promotion and termination of RNA transcription.

2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

3. Pilus genes of Neisseria gonorrheae: chromosomal organization and DNA sequence.

4. The spontaneous insertion of proteins into and across membranes: the helical hairpin hypothesis.

5. Nucleotide sequence of the gene encoding pilin of Bacteroides nodosus, the causal organism of ovine footrot.

6. Repetitive extragenic palindromic sequences: a major component of the bacterial genome.

7. A comprehensive sequence analysis program for the IBM personal computer.

8. Cytoplasmic membrane vesicles of Escherichia coli. A simple method for preparing the cytoplasmic and outer membranes.

9. Identification of pilin pools in the membranes of Pseudomonas aeruginosa.

10. A function of Pseudomonas aeruginosa PAO polar pili: twitching motility.

1. Overproduction of an acetylxylan esterase from the extreme thermophile "Caldocellum saccharolyticum" in Escherichia coli.

2. Type IV pilus retraction in pathogenic Neisseria is regulated by the PilC proteins.

3. Characterization of the genome of Pseudomonas aeruginosa bacteriophage phi PLS27 with particular reference to the ends of the DNA.

4. Cloning and expression of a Salmonella enteritidis fimbrin gene in Escherichia coli.

5. An analysis of the organization and evolution of type 4 fimbrial (MePhe) subunit proteins.

6. Cloning and expression of Pseudomonas aeruginosa flagellin in Escherichia coli.

7. Complete nucleotide sequence of the structural gene for alkaline proteinase from Pseudomonas aeruginosa IFO 3455.

8. Production of Neisseria gonorrhoeae pili (fimbriae) in Pseudomonas aeruginosa.

9. Neisseria gonorrhoeae prepilin export studied in Escherichia coli.

10. Functional characterization of bacterial oligosaccharyltransferases involved in O-linked protein glycosylation.