Literature DB >> 6793035

Isolation and characterization of a myeloma--spleen-cell hybrid producing antibody to phenylalanine hydroxylase.

R G Cotton, I G Jennings, K H Choo, K Fowler.   

Abstract

Application of the technique of myeloma--spleen-cell fusion [Kohler & Milstein (1975) Nature (London) 256, 495--497] has allowed the isolation of a cell colony that produced a monoclonal antibody against monkey liver phenylalanine hydroxylase. The antibody exhibited cross-reactivity against hepatic phenylalanine hydroxylase from other mammalian species, including human, rat and mouse. Cross-reactivity was established by (a) enzyme-inhibition assay, (b) double-immunodiffusion reaction, and (c) two-dimensional polyacrylamide-gel-electrophoretic analysis of immunoprecipitate. The various properties of the monoclonal antibody and its use in the study of mammalian phenylalanine hydroxylase are presented.

Entities:  

Mesh：

Substances：

Year:  1980        PMID： 6793035      PMCID： PMC1162277          DOI： 10.1042/bj1910777

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  10 in total

1.  Continuous cultures of fused cells secreting antibody of predefined specificity.

Authors:  G Köhler; C Milstein
Journal:  Nature       Date:  1975-08-07       Impact factor: 49.962

2.  Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.

Authors:  R A Laskey; A D Mills
Journal:  Eur J Biochem       Date:  1975-08-15

3.  Letter: Fusion of two immunoglobulin-producing myeloma cells.

Authors:  R G Cotton; C Milstein
Journal:  Nature       Date:  1973-07-06       Impact factor: 49.962

4.  A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

Authors:  W M Bonner; R A Laskey
Journal:  Eur J Biochem       Date:  1974-07-01

5.  Antibodies to major histocompatibility antigens produced by hybrid cell lines.

Authors:  G Galfre; S C Howe; C Milstein; G W Butcher; J C Howard
Journal:  Nature       Date:  1977-04-07       Impact factor: 49.962

6.  Genetics of the mammalian phenylalanine hydroxylase system. II. Immunological and two-dimensional gel electrophoretic studies of phenylalanine hydroxylase in cultured normal and mutant rat hepatoma cells.

Authors:  K H Choo; R G Cotton
Journal:  Biochem Genet       Date:  1979-10       Impact factor: 1.890

7.  Fusion between immunoglobulin-secreting and nonsecreting myeloma cell lines.

Authors:  G Köhler; S C Howe; C Milstein
Journal:  Eur J Immunol       Date:  1976-04       Impact factor: 5.532

8.  Phenylalanine hydroxylase of Macaca irus A simple purification by affinity chromatography.

Authors:  R G Cotton; P J Grattan
Journal:  Eur J Biochem       Date:  1975-12-15

9.  Genetics of the mammalian phenylalanine hydroxylase system. Studies of human liver phenylalanine hydroxylase subunit structure and of mutations in phenylketonuria.

Authors:  K H Choo; R G Cotton; D M Danks; I G Jennings
Journal:  Biochem J       Date:  1979-08-01       Impact factor: 3.857

10.  Observations indicating the nature of the mutation in phenylketonuria.

Authors:  K H Choo; R G Cotton; I G Jennings; D M Danks
Journal:  J Inherit Metab Dis       Date:  1980       Impact factor: 4.982
  10 in total
  9 in total

1.  Functional analysis of the effect of monoclonal antibodies on monkey liver phenylalanine hydroxylase.

Authors:  I G Jennings; R G Russell; W L Armarego; R G Cotton
Journal:  Biochem J       Date:  1986-04-01       Impact factor: 3.857

2.  Neutralizing monoclonal antibodies to human rotavirus and indications of antigenic drift among strains from neonates.

Authors:  B S Coulson; K J Fowler; R F Bishop; R G Cotton
Journal:  J Virol       Date:  1985-04       Impact factor: 5.103

3.  Monoclonal antibodies that immunoreact with a cation-stimulated plant membrane ATPase.

Authors:  J J Chin
Journal:  Biochem J       Date:  1982-04-01       Impact factor: 3.857

Review 4.  Hybridomas: a new dimension in biological analyses.

Authors:  R H Kennett
Journal:  In Vitro       Date:  1981-12

5.  Comparative studies of four monoclonal antibodies to phenylalanine hydroxylase exhibiting different properties with respect to substrate-dependence, species-specificity and a range of effects on enzyme activity.

Authors:  K H Choo; I G Jennings; R G Cotton
Journal:  Biochem J       Date:  1981-12-01       Impact factor: 3.857

6.  Melatonin Synthesis: Acetylserotonin O-Methyltransferase (ASMT) Is Strongly Expressed in a Subpopulation of Pinealocytes in the Male Rat Pineal Gland.

Authors:  Martin F Rath; Steven L Coon; Fernanda G Amaral; Joan L Weller; Morten Møller; David C Klein
Journal:  Endocrinology       Date:  2016-03-07       Impact factor: 4.736

7.  Interaction with a monoclonal antibody alters the expression of co-operativity by phenylalanine hydroxylase from rat liver.

Authors:  M A Parniak; I G Jennings; R G Cotton
Journal:  Biochem J       Date:  1989-01-15       Impact factor: 3.857

8.  Identification of two molecular-mass forms of phenylalanine hydroxylase that segregate independently in rats. Specific association of each form with certain rat strains.

Authors:  J F Mercer; A Grimes; I Jennings; R G Cotton
Journal:  Biochem J       Date:  1984-05-01       Impact factor: 3.857

9.  A novel two-dimensional polyacrylamide-gel pattern, which may be due to allelic genes, of phenylalanine hydroxylase in monkeys.

Authors:  S C Smith; W McAdam; R G Cotton; J F Mercer
Journal:  Biochem J       Date:  1985-10-01       Impact factor: 3.857
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.