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Literature DB >> 3933488

A novel two-dimensional polyacrylamide-gel pattern, which may be due to allelic genes, of phenylalanine hydroxylase in monkeys.

S C Smith, W McAdam, R G Cotton, J F Mercer.

Abstract

Two-dimensional gel electrophoresis of immunopurified monkey liver phenylalanine hydroxylase showed a novel form of the enzyme, in 4 out of 24 monkeys, in which each polypeptide spot was split into a doublet with the same charge but slightly different mobility in the sodium dodecyl sulphate/polyacrylamide-gel-electrophoresis (as opposed to the isoelectric-focusing) dimension. Phenylalanine hydroxylase formed by translation of RNA from a liver containing the novel form showed the doublet pattern, suggesting that it is due to differences in mRNA. By analogy with the rat, this mRNA difference could be due to allelic genes.

Entities: Chemical Gene Species

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Year: 1985 PMID： 3933488 PMCID： PMC1152723 DOI： 10.1042/bj2310197

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

12 in total

1. High resolution two-dimensional electrophoresis of proteins.

Authors: P H O'Farrell
Journal: J Biol Chem Date: 1975-05-25 Impact factor: 5.157

2. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

Authors: W M Bonner; R A Laskey
Journal: Eur J Biochem Date: 1974-07-01

3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

4. Comparative studies of four monoclonal antibodies to phenylalanine hydroxylase exhibiting different properties with respect to substrate-dependence, species-specificity and a range of effects on enzyme activity.

Authors: K H Choo; I G Jennings; R G Cotton
Journal: Biochem J Date: 1981-12-01 Impact factor: 3.857

5. Induction of metallothionein mRNA in rat liver and kidney after copper chloride injection.

Authors: S A Wake; J F Mercer
Journal: Biochem J Date: 1985-06-01 Impact factor: 3.857

6. Genetics of the mammalian phenylalanine hydroxylase system. Studies of human liver phenylalanine hydroxylase subunit structure and of mutations in phenylketonuria.

Authors: K H Choo; R G Cotton; D M Danks; I G Jennings
Journal: Biochem J Date: 1979-08-01 Impact factor: 3.857

7. The two molecular weight forms of rat phenylalanine hydroxylase are encoded by different messenger RNAs.

Authors: J F Mercer; S M Hunt; R G Cotton
Journal: J Biol Chem Date: 1983-05-10 Impact factor: 5.157

8. Isolation and characterization of a myeloma--spleen-cell hybrid producing antibody to phenylalanine hydroxylase.

Authors: R G Cotton; I G Jennings; K H Choo; K Fowler
Journal: Biochem J Date: 1980-12-01 Impact factor: 3.857

9. Identification of two molecular-mass forms of phenylalanine hydroxylase that segregate independently in rats. Specific association of each form with certain rat strains.

Authors: J F Mercer; A Grimes; I Jennings; R G Cotton
Journal: Biochem J Date: 1984-05-01 Impact factor: 3.857