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Literature DB >> 678298

Messenger RNA for the insect storage protein calliphorin: in vitro translation and chromosomal hybridization analyses of a 20 S poly(A)-RNA fraction.

D J Kemp, J A Thomson, W J Peacock, T J Higgins.

Abstract

A major poly(A)-containing RNA fraction of the approximate size expected of a monocistronic mRNA for the storage protein calliphorin has been isolated from the larval fat bodies of Calliphora vicina during early instar 3. This 20 S RNA fraction programs the synthesis by cell-free wheat embryo extracts of polypeptides of 86,000 daltons identified by tryptic peptide fingerprinting as precursors of the authentic calliphorin subunits of 83,000 daltons. Complementary DNA synthesized by AMV reverse transcriptase using the same 20 S RNA as template hybridized in situ to a single segment of one or two bands in the salivary polytene chromosomes of C. vicina.

Entities: Chemical Species

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Year: 1978 PMID： 678298 DOI： 10.1007/BF00484091

Source DB: PubMed Journal: Biochem Genet ISSN： 0006-2928 Impact factor: 1.890

25 in total

1. Electrophoretic patterns of deadenylylated chorion and globin mRNAs.

Authors: J N Vournakis; A Efstratiadis; F C Kafatos
Journal: Proc Natl Acad Sci U S A Date: 1975-08 Impact factor: 11.205

2. Occurrence of short particles in beans infected with the cowpea strain of TMV. II. Evidence that short particles contain the cistron for coat-protein.

Authors: T J Higgins; P B Goodwin; P R Whitfeld
Journal: Virology Date: 1976-06 Impact factor: 3.616

3. Occurrence of heat-dissociable ribosomal RNA in insects: the presence of three polynucleotide chains in 26 S RNA from cultured Aedes aegypti cells.

Authors: J Shine; L Dalgarno
Journal: J Mol Biol Date: 1973-03-25 Impact factor: 5.469

4. Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ.

Authors: B E Roberts; B M Paterson
Journal: Proc Natl Acad Sci U S A Date: 1973-08 Impact factor: 11.205

5. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

Authors: W M Bonner; R A Laskey
Journal: Eur J Biochem Date: 1974-07-01

6. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms.

Authors: R J Britten; D E Kohne
Journal: Science Date: 1968-08-09 Impact factor: 47.728

1. Identification and molecular analysis of a multigene family encoding calliphorin, the major larval serum protein of Calliphora vicina.

Authors: H Schenkel; J Kejzlarová-Lepesant; P Berreur; J Moreau; K Scheller; F Brègègére; J A Lepesant
Journal: EMBO J Date: 1985-11 Impact factor: 11.598

1 in total

Messenger RNA for the insect storage protein calliphorin: in vitro translation and chromosomal hybridization analyses of a 20 S poly(A)-RNA fraction.

1. Electrophoretic patterns of deadenylylated chorion and globin mRNAs.

2. Occurrence of short particles in beans infected with the cowpea strain of TMV. II. Evidence that short particles contain the cistron for coat-protein.

3. Occurrence of heat-dissociable ribosomal RNA in insects: the presence of three polynucleotide chains in 26 S RNA from cultured Aedes aegypti cells.

4. Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ.

5. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

6. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms.

7. The interpretation of puff patterns in polytene chromosomes.

8. Unique and repetitive sequences in multiple genes for feather keratin.

9. Genetics of lucilin, a storage protein from the sheep blowfly, Lucilia cuprina (Calliphoridae).

10. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

1. Identification and molecular analysis of a multigene family encoding calliphorin, the major larval serum protein of Calliphora vicina.