Literature DB >> 1059086

Electrophoretic patterns of deadenylylated chorion and globin mRNAs.

J N Vournakis, A Efstratiadis, F C Kafatos.   

Abstract

Treatment of silkmoth chorion mRNAs with calf thymus RNase H (EC 3.1.4.34; RNA-DNA-hybrid ribonucleotidohydrolase) in the presence of oligo(dT) specifically and effectively removes the 3'-terminal poly(A) sequences. Excision of non-poly(A) fragments cannot be detected. Under these conditions, RNase H leads to increased electrophoretic homogeneity of rabbit globin mRNA, presumably as a result of removal of poly(A) sequences that are inherently variable in length. Treatment with RNase H converts the three diffuse zones of messages for the several chorion proteins into multiple sharp bands.

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Year:  1975        PMID： 1059086      PMCID： PMC432898          DOI： 10.1073/pnas.72.8.2959

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  20 in total

Review 1.  Organization, transcription, and regulation in the animal genome.

Authors:  E H Davidson; R J Britten
Journal:  Q Rev Biol       Date:  1973-12       Impact factor: 4.875

2.  In vitro translation of polyadenylic acid-free rabbit globin messenger RNA.

Authors:  H Soreq; U Nudel; R Salomon; M Revel; U Z Littauer
Journal:  J Mol Biol       Date:  1974-09-05       Impact factor: 5.469

3.  The separation of alpha- and beta-rabbit globin mRNA by polyacrylamide gel electrophoresis.

Authors:  A Berns; P Jansen; H Bloemendal
Journal:  FEBS Lett       Date:  1974-10-15       Impact factor: 4.124

4.  Size heterogeneity of polyadenylate sequences in mouse globin messenger RNA.

Authors:  J Gorski; M R Morrison; C G Merkel; J B Lingrel
Journal:  J Mol Biol       Date:  1974-06-25       Impact factor: 5.469

5.  Sequence at the 3' end of globin mRNA shows homology with immunoglobulin light chain mRNA.

Authors:  N J Proudfoot; G G Brownlee
Journal:  Nature       Date:  1974-11-29       Impact factor: 49.962

6.  Translation of mouse globin messenger ribonucleic acid from which the poly(adenylic acid) sequence has been removed.

Authors:  R Williamson; J Crossley; S Humphries
Journal:  Biochemistry       Date:  1974-02-12       Impact factor: 3.162

7.  Translational properties of rabbit globin mRNA after specific removal of poly(A) with ribonuclease H.

Authors:  A E Sippel; J G Stavrianopoulos; G Schutz; P Feigelson
Journal:  Proc Natl Acad Sci U S A       Date:  1974-11       Impact factor: 11.205

8.  Purification of a family of specific messenger ribonucleic acids from moth follicular cells.

Authors:  R E Gelinas; F C Kafatos
Journal:  Proc Natl Acad Sci U S A       Date:  1973-12       Impact factor: 11.205

9.  The molecular weight of rabbit globin messenger RNA's.

Authors:  H J Gould; P H Hamlyn
Journal:  FEBS Lett       Date:  1973-03-15       Impact factor: 4.124

10.  Full length and discrete partial reverse transcripts of globin and chorion mRNAs.

Authors:  A Efstratiadis; T Maniatis; F C Kafatos; A Jeffrey; J N Vournakis
Journal:  Cell       Date:  1975-04       Impact factor: 41.582
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  26 in total

1.  Newcastle disease virus-specific RNA: poly(A)-containing and poly(A)-deficient transcripts as revealed by chromatography on poly(U)-sepharose.

Authors:  N L Varich; I S Lukashevich; N V Kaverin
Journal:  J Virol       Date:  1976-04       Impact factor: 5.103

2.  Structure and transcription of a human gene for H1 RNA, the RNA component of human RNase P.

Authors:  M Baer; T W Nilsen; C Costigan; S Altman
Journal:  Nucleic Acids Res       Date:  1990-01-11       Impact factor: 16.971

3.  Messenger RNA deadenylylation precedes decapping in mammalian cells.

Authors:  P Couttet; M Fromont-Racine; D Steel; R Pictet; T Grange
Journal:  Proc Natl Acad Sci U S A       Date:  1997-05-27       Impact factor: 11.205

4.  Specific hydrolysis of rabbit globin messenger RNA by S1 nuclease.

Authors:  M S Flashner; J N Vournakis
Journal:  Nucleic Acids Res       Date:  1977-07       Impact factor: 16.971

5.  Glucocorticoids enhance stability of human growth hormone mRNA.

Authors:  I Paek; R Axel
Journal:  Mol Cell Biol       Date:  1987-04       Impact factor: 4.272

6.  Differential expression of opioid peptide genes by testicular germ cells and somatic cells.

Authors:  D L Kilpatrick; K Borland; D F Jin
Journal:  Proc Natl Acad Sci U S A       Date:  1987-08       Impact factor: 11.205

7.  Tandemly repeated exons encode 81-base repeats in multiple, developmentally regulated Schistosoma mansoni transcripts.

Authors:  R E Davis; A H Davis; S M Carroll; A Rajkovic; F M Rottman
Journal:  Mol Cell Biol       Date:  1988-11       Impact factor: 4.272

8.  Rat growth hormone gene: intervening sequences separate the mRNA regions.

Authors:  H Soreq; M Harpold; R Evans; J E Darnell; F C Bancroft
Journal:  Nucleic Acids Res       Date:  1979-06-11       Impact factor: 16.971

9.  Multiple polymorphic alpha- and beta-tubulin mRNAs are present in sea urchin eggs.

Authors:  D Alexandraki; J V Ruderman
Journal:  Proc Natl Acad Sci U S A       Date:  1985-01       Impact factor: 11.205

10.  Tissue-specific expression of insulin-like growth factor II mRNAs with distinct 5' untranslated regions.

Authors:  J C Irminger; K M Rosen; R E Humbel; L Villa-Komaroff
Journal:  Proc Natl Acad Sci U S A       Date:  1987-09       Impact factor: 11.205
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