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Literature DB >> 6769970

Interlaboratory comparison of serum vitamin B12 assay.

D L Mollin, A V Hoffbrand, P G Ward, S M Lewis.

Abstract

The results have been compared of microbiological and radioisotope dilution (RID) assay of serum vitamin B12 by participants in national interlaboratory trials in Britain. There was wide variation between the individual participants, especially marked in the L. leichmannii microbiological assay and in the RID methods, whereas excellent correlation, reproducibility, and recovery were obtained in reference laboratories by microbiological assay with both E. gracilis and L. leichmannii. In general, RID gave higher results than microbiological assay. The need for suitable reference sera is emphasised.

Entities: Chemical Disease Species

Mesh：

Substances：
Vitamin B 12

Year: 1980 PMID： 6769970 PMCID： PMC1146047 DOI： 10.1136/jcp.33.3.243

Source DB: PubMed Journal: J Clin Pathol ISSN： 0021-9746 Impact factor: 3.411

14 in total

1. Improved method for measuring vitamin B12 in serum using intrinsic factor, 57CoB12, and coated charcoal.

Authors: J L Raven; M B Robson; P L Walker; P Barkhan
Journal: J Clin Pathol Date: 1969-03 Impact factor: 3.411

2. A radiosorbent technique for the assay of serum vitamin B12.

Authors: L Wide; A Killander
Journal: Scand J Clin Lab Invest Date: 1971-04 Impact factor: 1.713

3. Reliability of radioisotope-dilution kits for vitamin B.

Authors: C A Hall
Journal: Lancet Date: 1977-06-11 Impact factor: 79.321

4. Pernicious anemia with neuromyelopathy and normal serum-vitamin-B12.

Authors: J M Mahood
Journal: Lancet Date: 1977-11-05 Impact factor: 79.321

5. The use of chicken serum for measurement of serum vitamin B12 concentration by radioisotope dilution: discription of method and comparison with microbiological assay results.

Authors: R Green; P A Newmark; A M Musso; D L Mollin
Journal: Br J Haematol Date: 1974-07 Impact factor: 6.998

6. Comparison of three methods for measuring vitamin B 12 in serum: radioisotopic, euglena gracilis and Lactobacillus leichmannii.

Authors: J L Raven; M B Robson; J O Morgan; A V Hoffbrand
Journal: Br J Haematol Date: 1972-01 Impact factor: 6.998

7. Absorption, plasma transport, and cellular retention of cobalamin analogues in the rabbit. Evidence for the existence of multiple mechanisms that prevent the absorption and tissue dissemination of naturally occurring cobalamin analogues.

Authors: J F Kolhouse; R H Allen
Journal: J Clin Invest Date: 1977-12 Impact factor: 14.808

Review 8. The serum vitamin B12 level: its assay and significance.

Authors: D L Mollin; B B Anderson; J F Burman
Journal: Clin Haematol Date: 1976-10

9. Experience with a commercial kit for the radioisotopic assay of vitamin B12 in serum: the Phadebas B12 test.

Authors: J L Raven; M B Robson
Journal: J Clin Pathol Date: 1974-01 Impact factor: 3.411

10. Cobalamin analogues are present in human plasma and can mask cobalamin deficiency because current radioisotope dilution assays are not specific for true cobalamin.

Authors: J F Kolhouse; H Kondo; N C Allen; E Podell; R H Allen
Journal: N Engl J Med Date: 1978-10-12 Impact factor: 91.245

5 in total

1. Development of a specific radioimmunoassay for vitamin B12 and its application in the diagnosis of cobalt deficiency in sheep.

Authors: D G Kennedy; F P O'Harte; W J Blanchflower; D A Rice
Journal: Vet Res Commun Date: 1990 Impact factor: 2.459