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Literature DB >> 5776552

Improved method for measuring vitamin B12 in serum using intrinsic factor, 57CoB12, and coated charcoal.

J L Raven, M B Robson, P L Walker, P Barkhan.

Abstract

An improved and simplified method is described for the measurement of vitamin B(12) in serum using intrinsic factor, (57)CoB(12), and coated charcoal. The extraction of serum in the presence of cyanide and the incorporation of B(12)-deficient serum into the intrinsic factor control has increased the accuracy of the method for both sera and crystalline B(12) solutions. There are interesting differences between the results obtained for some sera by the isotope and L. leichmannii methods and the reasons for these differences are discussed.

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Year: 1969 PMID： 5776552 PMCID： PMC474034 DOI： 10.1136/jcp.22.2.205

Source DB: PubMed Journal: J Clin Pathol ISSN： 0021-9746 Impact factor: 3.411

8 in total

1. Assay of vitamin B12 in blood. A simple method.

Authors: R M BARAKAT; R P EKINS
Journal: Lancet Date: 1961-07-01 Impact factor: 79.321

2. MEASUREMENT OF SERUM VITAMIN B12 LEVEL USING RADIOISOTOPE DILUTION AND COATED CHARCOAL.

Authors: K S LAU; C GOTTLIEB; L R WASSERMAN; V HERBERT
Journal: Blood Date: 1965-08 Impact factor: 22.113

3. Isotopic determination of vitamin B12 binding capacity and concentration.

Authors: N GROSSOWICZ; D SULITZEANU; D MERZBACH
Journal: Proc Soc Exp Biol Med Date: 1962-03

4. An improved method for the rapid estimation of vitamin B12 in serum.

Authors: G H SPRAY
Journal: Clin Sci Date: 1955-11 Impact factor: 6.124

5. The determination of vitamin B12 activity in human serum.

Authors: H L ROSENTHAL; H P SARETT
Journal: J Biol Chem Date: 1952-11 Impact factor: 5.157

6. Comparison of the radioisotope dilution-coated charcoal method and a microbiological method (L. leichmannii) for measuring vitamin B12 in serum.

Authors: J L Raven; P L Walker; P Barkhan
Journal: J Clin Pathol Date: 1966-11 Impact factor: 3.411

7. Results with radioisotopic assay of serum B12 using serum binding agent.

Authors: D M Matthews; R Gunasegaram; J C Linnell
Journal: J Clin Pathol Date: 1967-09 Impact factor: 3.411

8. Radioisotopic assay of serum vitamin B 12 with the use of DEAE cellulose.

Authors: E P Frenkel; S Keller; M S McCall
Journal: J Lab Clin Med Date: 1966-09

8 in total

11 in total

1. The effect of autoclaving characteristics on the recovery of serum vitamin B 12 determined by a radioisotope dilution method.

Authors: D H Orrell; A D Caswell
Journal: J Clin Pathol Date: 1972-02 Impact factor: 3.411

2. Assay of vitamin B 12 .

Authors: J L Raven; P Barkhan
Journal: J Clin Pathol Date: 1972-12 Impact factor: 3.411

3. Vitamin B 12 binding by intrinsic factor.

Authors: M S Rose
Journal: J Clin Pathol Date: 1971-11 Impact factor: 3.411

4. Effect of pH changes on the binding of vitamin B12 by intrinsic factor.

Authors: H Y Shum; B J O'Neill; A M Streeter
Journal: J Clin Pathol Date: 1971-04 Impact factor: 3.411

5. Underestimation of vitamin B 12 in serum by a microbiological method(Lactobacillus leichmannii): comparison with a radioisotopic method.

Authors: J L Raven; M B Robson; P Barkhan
Journal: J Clin Pathol Date: 1969-11 Impact factor: 3.411