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Literature DB >> 4821096

Experience with a commercial kit for the radioisotopic assay of vitamin B12 in serum: the Phadebas B12 test.

Abstract

The first commercial kit for the radioisotopic assay of vitamin B(12) in serum-the Phadebas B(12) Test produced higher values than the radioisotopic method of Raven, Robson, Walker, and Barkham (1969) and the Lactobacillus leichmannii microbiological assay. Its normal range was 300-1100 pg/ml and its reproducibility was similar to that of the other radioisotopic method. It should be possible to lower the results obtained by the Phadebas method by modifying its standard curve and to reduce the time taken for the assay by shortening its incubation period.

Entities: Chemical Species

Mesh：

Substances：

Year: 1974 PMID： 4821096 PMCID： PMC477988 DOI： 10.1136/jcp.27.1.59

Source DB: PubMed Journal: J Clin Pathol ISSN： 0021-9746 Impact factor: 3.411

12 in total

1. An improved method for the rapid estimation of vitamin B12 in serum.

Authors: G H SPRAY
Journal: Clin Sci Date: 1955-11 Impact factor: 6.124

2. The determination of vitamin B12 activity in human serum.

Authors: H L ROSENTHAL; H P SARETT
Journal: J Biol Chem Date: 1952-11 Impact factor: 5.157

3. The effect of cyanide, serum and other factors on the assay of vitamin B12 by a radio-isotope method using 57CoB12, intrinsic factor and coated charcoal.

Authors: J L Raven; M B Robson; P L Walker; P Barkhan
Journal: Guys Hosp Rep Date: 1968

4. Negative interference by residual proteins in the supernatant fluid used in radioisotopic assay of serum vitamin B 12 .

Authors: E Mortensen
Journal: Clin Chem Date: 1972-09 Impact factor: 8.327

5. Comparison of two radioassay methods for vitamin B 12 .

Authors: N P Kubasik; M H Murray
Journal: Clin Chem Date: 1972-07 Impact factor: 8.327

6. Hemoglobin-coated charcoal radioassay for serum vitamin B12. A simple modification to improve intrinsic factor reliability.

Authors: R S Hillman; M Oakes; C Finholt
Journal: Blood Date: 1969-09 Impact factor: 22.113

7. Improved method for measuring vitamin B12 in serum using intrinsic factor, 57CoB12, and coated charcoal.

Authors: J L Raven; M B Robson; P L Walker; P Barkhan
Journal: J Clin Pathol Date: 1969-03 Impact factor: 3.411

8. A radiosorbent technique for the assay of serum vitamin B12.

Authors: L Wide; A Killander
Journal: Scand J Clin Lab Invest Date: 1971-04 Impact factor: 1.713

9. Comparison of three methods for measuring vitamin B 12 in serum: radioisotopic, euglena gracilis and Lactobacillus leichmannii.

Authors: J L Raven; M B Robson; J O Morgan; A V Hoffbrand
Journal: Br J Haematol Date: 1972-01 Impact factor: 6.998

6. Comparison of serum vitamin B12 estimation by saturation analysis with intrinsic factor and with R-protein as binding agents.

Authors: R Zacharakis; M Muir; I Chanarin
Journal: J Clin Pathol Date: 1981-04 Impact factor: 3.411

6 in total

Experience with a commercial kit for the radioisotopic assay of vitamin B12 in serum: the Phadebas B12 test.

1. An improved method for the rapid estimation of vitamin B12 in serum.

2. The determination of vitamin B12 activity in human serum.

3. The effect of cyanide, serum and other factors on the assay of vitamin B12 by a radio-isotope method using 57CoB12, intrinsic factor and coated charcoal.

4. Negative interference by residual proteins in the supernatant fluid used in radioisotopic assay of serum vitamin B 12 .

5. Comparison of two radioassay methods for vitamin B 12 .

6. Hemoglobin-coated charcoal radioassay for serum vitamin B12. A simple modification to improve intrinsic factor reliability.

7. Improved method for measuring vitamin B12 in serum using intrinsic factor, 57CoB12, and coated charcoal.

8. A radiosorbent technique for the assay of serum vitamin B12.

9. Comparison of three methods for measuring vitamin B 12 in serum: radioisotopic, euglena gracilis and Lactobacillus leichmannii.

10. Addisonian pernicious anaemia and intrinsic factor antibodies in thyroid disorders.

Review 1. Megaloblastic anaemia, cobalamin, and folate.

2. Interlaboratory comparison of serum vitamin B12 assay.

3. The influence of serum folate on urinary excretion of vitamin B12.

4. An evaluation of commercial radioisotope methods for the determination of folate and vitamin B12.

5. Extraction of serum vitamin B12 for radio-isotopic and Lactobacillus leichmannii assay.

6. Comparison of serum vitamin B12 estimation by saturation analysis with intrinsic factor and with R-protein as binding agents.