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Literature DB >> 6769902

Plasmid-mediated transformation in Bacillus megaterium.

Abstract

A transformation system was developed for Bacillus megaterium by using antibiotic resistance plasmid deoxyribonucleic acid molecules derived from Staphylococcus aureus and Bacillus cereus. Lysozyme-generated protoplasts of B. megaterium allowed uptake of plasmid deoxyribonucleic acid in the presence of polyethylene glycol. Transformants expressed the antibiotic resistance determinants present on the plasmid deoxyribonucleic acid, and reisolated plasmid deoxyribonucleic acid yielded restriction endonuclease digestion patterns identical to those of the donor deoxyribonucleic acid.

Entities: Chemical Species

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Year: 1980 PMID： 6769902 PMCID： PMC294013 DOI： 10.1128/jb.142.2.508-512.1980

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

10 in total

1. Physical mapping of a plasmid from Bacillus megaterium by restriction endonuclease cleavage.

Authors: B C Carlton; B J Brown
Journal: Plasmid Date: 1979-01 Impact factor: 3.466

2. Interspecific induction of transformation of bacteria with respect to phage-susceptibility.

Authors: S FUKUDA; K TSUZUKI
Journal: Nature Date: 1961-06-03 Impact factor: 49.962

3. Transformation in Bacillus subtilis. Fate of newly introduced transforming DNA.

Authors: F Arwert; G Venema
Journal: Mol Gen Genet Date: 1973

4. Fusion of bacterial protoplasts.

Authors: P Schaeffer; B Cami; R D Hotchkiss
Journal: Proc Natl Acad Sci U S A Date: 1976-06 Impact factor: 11.205

5. High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA.

Authors: S Chang; S N Cohen
Journal: Mol Gen Genet Date: 1979-01-05

6. Polyethylene-glycol induced fusion of bacterial protoplasts: direct selection of recombinants.

Authors: K Fodor; L Alföldi
Journal: Mol Gen Genet Date: 1979-01-05

7. Characterization of the polydisperse closed circular deoxyribonucleic acid molecules of Bacillus megaterium.

Authors: R C Henneberry; B C Carlton
Journal: J Bacteriol Date: 1973-05 Impact factor: 3.490

8. Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis.

Authors: T J Gryczan; S Contente; D Dubnau
Journal: J Bacteriol Date: 1978-04 Impact factor: 3.490

9. Construction and properties of chimeric plasmids in Bacillus subtilis.

Authors: T J Gryczan; D Dubnau
Journal: Proc Natl Acad Sci U S A Date: 1978-03 Impact factor: 11.205

10. Bacteriocin and antibiotic resistance plasmids in Bacillus cereus and Bacillus subtilis.

Authors: K Bernhard; H Schrempf; W Goebel
Journal: J Bacteriol Date: 1978-02 Impact factor: 3.490

10 in total

19 in total

1. Efficient transformation of Bacillus thuringiensis and B. cereus via electroporation: transformation of acrystalliferous strains with a cloned delta-endotoxin gene.

Authors: W Schurter; M Geiser; D Mathé
Journal: Mol Gen Genet Date: 1989-07

2. Transformation of Streptococcus lactis Protoplasts by Plasmid DNA.

Authors: J K Kondo; L L McKay
Journal: Appl Environ Microbiol Date: 1982-05 Impact factor: 4.792

3. Use of bacterial luciferase to establish a promoter probe vehicle capable of nondestructive real-time analysis of gene expression in Bacillus spp.

Authors: O A Carmi; G S Stewart; S Ulitzur; J Kuhn
Journal: J Bacteriol Date: 1987-05 Impact factor: 3.490

4. Complete nucleotide sequences of Bacillus plasmids pUB110dB, pRBH1 and its copy mutants.

Authors: R E Muller; T Ano; T Imanaka; S Aiba
Journal: Mol Gen Genet Date: 1986-01

5. Isolation and genetic characterizations of Bacillus megaterium cobalamin biosynthesis-deficient mutants.

Authors: J B Wolf; R N Brey
Journal: J Bacteriol Date: 1986-04 Impact factor: 3.490

6. Molecular cloning of structural and immunity genes for megacins A-216 and A-19213 in Bacillus megaterium.

Authors: M A Von Tersch; B C Carlton
Journal: J Bacteriol Date: 1984-12 Impact factor: 3.490

7. Role and Function of LitR, an Adenosyl B12-Bound Light-Sensitive Regulator of Bacillus megaterium QM B1551, in Regulation of Carotenoid Production.

Authors: Hideaki Takano; Kou Mise; Kenta Hagiwara; Naoya Hirata; Shoko Watanabe; Minami Toriyabe; Hatsumi Shiratori-Takano; Kenji Ueda
Journal: J Bacteriol Date: 2015-04-27 Impact factor: 3.490