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Literature DB >> 4196248

Characterization of the polydisperse closed circular deoxyribonucleic acid molecules of Bacillus megaterium.

Abstract

The polydisperse circular deoxyribonucleic acid (DNA) molecules which comprise up to 30% of the total extractable DNA of Bacillus megaterium strain 216 have been purified and partially characterized. Banding in cesium chlorideethidium bromide by "gradient relaxation" in a fixed-angle rotor provided good resolution of circular and chromosomal DNAs for preparative separations. Renaturation studies on purified circular DNA failed to reveal a rapidly renaturing fraction, and DNA-DNA hybridization studies indicated that the majority of the chromosomal nucleotide sequences are represented in the heterogeneous-size population of circular molecules. It is concluded that the circular DNA of B. megaterium does not represent typical bacterial plasmid DNA. The possibility that the circular DNA molecules are the result of the expression of a defective bacteriophage is discussed.

Entities: Chemical Species

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Year: 1973 PMID： 4196248 PMCID： PMC251818 DOI： 10.1128/jb.114.2.625-631.1973

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

29 in total

1. Cultivation and electron microscopy of a bacteriocinogenic strain of Bacillus megaterium.

Authors: G IVANOVICS; L ALFOLDI; B LOVAS
Journal: Acta Microbiol Acad Sci Hung Date: 1957

2. Early and late helix-coil transitions in closed circular DNA. The number of superhelical turns in polyoma DNA.

Authors: J Vinograd; J Lebowitz; R Watson
Journal: J Mol Biol Date: 1968-04-14 Impact factor: 5.469

3. Density gradient relaxation: a method for preparative buoyant density separation of DNA.

Authors: R Anet; D R Strayer
Journal: Biochem Biophys Res Commun Date: 1969-02-07 Impact factor: 3.575

4. Defective bacteriophage PBSH in Bacillus subtilis. I. Induction, purification, and physical properties of the bacteriophage and its deoxyribonucleic acid.

Authors: M Haas; H Yoshikawa
Journal: J Virol Date: 1969-02 Impact factor: 5.103

5. Kinetics of renaturation of DNA.

Authors: J G Wetmur; N Davidson
Journal: J Mol Biol Date: 1968-02-14 Impact factor: 5.469

6. A quantitative assay for DNA-DNA hybrids using membrane filters.

Authors: S O Warmaar; J A Cohen
Journal: Biochem Biophys Res Commun Date: 1966-08-23 Impact factor: 3.575

7. Heterogeneous circular DNA elements in vegetative cultures of Bacillus megaterium.

Authors: B C Carlton; D R Helinski
Journal: Proc Natl Acad Sci U S A Date: 1969-10 Impact factor: 11.205

8. A membrane-filter technique for the detection of complementary DNA.

Authors: D T Denhardt
Journal: Biochem Biophys Res Commun Date: 1966-06-13 Impact factor: 3.575

9. Isolation of circular deoxyribonucleic acid from Salmonella typhosa hybrids obtained from matings with Escherichia coli Hfr donors.

Authors: R W Leavitt; J A Wohlhieter; E M Johnson; G E Olson; L S Baron
Journal: J Bacteriol Date: 1971-12 Impact factor: 3.490

5. Size distribution of the closed circular deoxyribonucleic acid molecules of Bacillus megaterium: sedimentation velocity and electron microscope measurements.

Authors: B C Carlton; M P Smith
Journal: J Bacteriol Date: 1974-03 Impact factor: 3.490

6. Physical characterization of a plasmid cointegrate containing an F'his gnd element and the Salmonella typhimurium LT2 cryptic plasmid.

Authors: J J Manis; H J Whitfield
Journal: J Bacteriol Date: 1977-03 Impact factor: 3.490

Characterization of the polydisperse closed circular deoxyribonucleic acid molecules of Bacillus megaterium.

1. Cultivation and electron microscopy of a bacteriocinogenic strain of Bacillus megaterium.

2. Early and late helix-coil transitions in closed circular DNA. The number of superhelical turns in polyoma DNA.

3. Density gradient relaxation: a method for preparative buoyant density separation of DNA.

4. Defective bacteriophage PBSH in Bacillus subtilis. I. Induction, purification, and physical properties of the bacteriophage and its deoxyribonucleic acid.

5. Kinetics of renaturation of DNA.

6. A quantitative assay for DNA-DNA hybrids using membrane filters.

7. Heterogeneous circular DNA elements in vegetative cultures of Bacillus megaterium.

8. A membrane-filter technique for the detection of complementary DNA.

9. Isolation of circular deoxyribonucleic acid from Salmonella typhosa hybrids obtained from matings with Escherichia coli Hfr donors.

10. Transcription of bacteriophage T4 deoxyribonucleic acid in vitro.

1. Evidence for a nonrandom base sequence in a Bacillus pumilus plasmid: EcoR1 endonuclease digestion of pPL576.

Review 2. Extrachromosomal elements as possible agents of adaptation and development.

3. New cryptic plasmid of Bacillus subtilis and restriction analysis of other plasmids found by general screening.

4. Isolation and characterization of plasmid from the Bacillus brevis var. G.-B. cells.

5. Size distribution of the closed circular deoxyribonucleic acid molecules of Bacillus megaterium: sedimentation velocity and electron microscope measurements.

6. Physical characterization of a plasmid cointegrate containing an F'his gnd element and the Salmonella typhimurium LT2 cryptic plasmid.

7. Plasmid deoxyribonucleic acid in Bacillus subtilis and Bacillus pumilus.

8. Megacinogenic plasmid from Bacillus megaterium 216.

9. Characterization of antigens from type A and B yeast cells of Histoplasma capsulatum.

10. Production of megacins C and Cx: presumptive evidence extrachromosomal control.