Literature DB >> 6769432

An accurate and sensitive analysis by high-pressure liquid chromatography of conjugated and unconjugated bilirubin IX-alpha in various biological fluids.

S Onishi, S Itoh, N Kawade, K Isobe, S Sugiyama.   

Abstract

An accurate and sensitive method was developed for the complete separation of the native tetrapyrroles, such as bilirubin and its mono- and di-conjugates of glucuronic acid, glucose and xylose, by ion-pair reversed-phase high-pressure liquid chromatography. The application of this method was demonstrated by the analysis of bile pigments in human bile and urine, and the method also makes it possible to estimate very low UDP-glucuronyltransferase activity, such as is found in the human foetal and neonatal liver.

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Year:  1980        PMID： 6769432      PMCID： PMC1161298          DOI： 10.1042/bj1850281

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  9 in total

1.  Photodegradation products of bilirubin studied by high pressure liquid chromatography, gel permeation chromatography, nuclear magnetic resonance and mass spectrometry.

Authors:  S Onishi; M Fujikake; Y Ogawa; J Ogawa
Journal:  Birth Defects Orig Artic Ser       Date:  1976

Review 2.  Twenty-five years of progress in bilirubin metabolism (1952-77).

Authors:  B H Billing
Journal:  Gut       Date:  1978-06       Impact factor: 23.059

3.  The isolation and characterization of bilirubin diglucuronide, the major bilirubin conjugate in dog and human bile.

Authors:  E R Gordon; C A Goresky; T H Chang; A S Perlin
Journal:  Biochem J       Date:  1976-06-01       Impact factor: 3.857

4.  Recent advances in the separation and analysis of diazo-positive bile pigments.

Authors:  K P Heirwegh; J Fevery; J A Meuwissen; J De Groote; F Compernolle; V Desmet; F P Van Roy
Journal:  Methods Biochem Anal       Date:  1974

5.  Determination of bilirubin UDP-glucuronyl transferase activity in needle-biopsy specimens of human liver.

Authors:  M Black; B H Billing; K P Heirwegh
Journal:  Clin Chim Acta       Date:  1970-07       Impact factor: 3.786

Review 6.  Critique of the assay and significance of bilirubin conjugation.

Authors:  K P Heirwegh; J A Meuwissen; J Fevery
Journal:  Adv Clin Chem       Date:  1973       Impact factor: 5.394

7.  Conjugated and unconjugated bilirubins in humans and rhesus monkeys. Structural identity of bilirubins from biles and meconiums of newborn humans and rhesus monkeys.

Authors:  S G Blumenthal; D B Taggart; R D Rasmussen; R M Ikeda; B H Ruebner; D E Bergstrom; F W Hanson
Journal:  Biochem J       Date:  1979-06-01       Impact factor: 3.857

8.  Determination of bilirubin glucuronide and assay of glucuronyltransferase with bilirubin as acceptor.

Authors:  F P Van Roy; K P Heirwegh
Journal:  Biochem J       Date:  1968-04       Impact factor: 3.857

9.  The isolation and further characterization of the bilirubin tetrapyrroles in bile-containing human duodenal juice and dog gall-bladder bile.

Authors:  E R Gordon; T H Chan; K Samodai; C A Goresky
Journal:  Biochem J       Date:  1977-10-01       Impact factor: 3.857
  9 in total
  7 in total

1.  High-pressure liquid chromatographic analysis of anaerobic photoproducts of bilirubin-IX alpha in vitro and its comparison with photoproducts in vivo.

Authors:  S Onishi; N Kawade; S Itoh; K Isobe; S Sugiyama
Journal:  Biochem J       Date:  1980-09-15       Impact factor: 3.857

2.  Demonstration of a geometric isomer of bilirubin-IX alpha in the serum of a hyperbilirubinaemic newborn infant and the mechanism of jaundice phototherapy.

Authors:  S Onishi; K Isobe; S Itoh; N Kawade; S Sugiyama
Journal:  Biochem J       Date:  1980-09-15       Impact factor: 3.857

3.  Investigation of the substrate specificity of a cloned expressed human bilirubin UDP-glucuronosyltransferase: UDP-sugar specificity and involvement in steroid and xenobiotic glucuronidation.

Authors:  S B Senafi; D J Clarke; B Burchell
Journal:  Biochem J       Date:  1994-10-01       Impact factor: 3.857

4.  Application of a rapid and efficient h.p.l.c. method to measure bilirubin and its conjugates from native bile and in model bile systems. Potential use as a tool for kinetic reactions and as an aid in diagnosis of hepatobiliary disease.

Authors:  W Spivak; W Yuey
Journal:  Biochem J       Date:  1986-02-15       Impact factor: 3.857

5.  Bilirubin diglucuronide formation in intact rats and in isolated Gunn rat liver.

Authors:  J R Chowdhury; N R Chowdhury; U Gärtner; A W Wolkoff; I M Arias
Journal:  J Clin Invest       Date:  1982-03       Impact factor: 14.808

Review 6.  Investigation of the molecular basis of the genetic deficiency of UDP-glucuronosyltransferase in Crigler-Najjar syndrome.

Authors:  K J Robertson; D Clarke; L Sutherland; R Wooster; M W Coughtrie; B Burchell
Journal:  J Inherit Metab Dis       Date:  1991       Impact factor: 4.982

7.  Reverse-phase h.p.l.c. separation, quantification and preparation of bilirubin and its conjugates from native bile. Quantitative analysis of the intact tetrapyrroles based on h.p.l.c. of their ethyl anthranilate azo derivatives.

Authors:  W Spivak; M C Carey
Journal:  Biochem J       Date:  1985-02-01       Impact factor: 3.857
  7 in total

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