Literature DB >> 6769112

ATP-dependent conjugation of reticulocyte proteins with the polypeptide required for protein degradation.

A Ciechanover, H Heller, S Elias, A L Haas, A Hershko.   

Abstract

The heat-stable polypeptide (APF-1) required for ATP-dependent proteolysis in reticulocytes enters into high molecular weight conjugates upon incubation with the fraction of reticulocytes that is retained by DEAE-cellulose. Conjugate formation requires ATP and Mg2+ and its inhibited by N-ethylmaleimide. UTP and GTP are inactive. These properties are identical to those of ATP-dependent protein breakdown in the same system, suggesting that the conjugates are intermediates in this process. The APF-1 conjugates are stable in sodium dodecyl sulfate/polyacrylamide gel electrophoresis and Sephadex G-75 isolation and are resistant to mild acid, alkali, heat denaturation, and reduction; the conjugates are therefore covalent.

Entities:  

Mesh：

Substances：

Year:  1980        PMID： 6769112      PMCID： PMC348495          DOI： 10.1073/pnas.77.3.1365

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  16 in total

1.  Requirement for protein synthesis in the regulation of protein breakdown in cultured hepatoma cells.

Authors:  D Epstein; S Elias-Bishko; A Hershko
Journal:  Biochemistry       Date:  1975-11-18       Impact factor: 3.162

2.  Intermediate steps in the degradation of a specific abnormal protein in Escherichia coli.

Authors:  J D Kowit; A L Goldberg
Journal:  J Biol Chem       Date:  1977-12-10       Impact factor: 5.157

Review 3.  Turnover of intracellular proteins.

Authors:  M J Pine
Journal:  Annu Rev Microbiol       Date:  1972       Impact factor: 15.500

4.  Influence of protease inhibitors and energy metabolism on intracellular protein breakdown in starving Escherichia coli.

Authors:  Y Shechter; D Rafaeli-Eshkol; A Hershko
Journal:  Biochem Biophys Res Commun       Date:  1973-10-15       Impact factor: 3.575

5.  Protein degradation in cultured cells. The effect of fresh medium, fluoride, and iodoacetate on the digestion of cellular protein of rat fibroblasts.

Authors:  B Poole; M Wibo
Journal:  J Biol Chem       Date:  1973-09-10       Impact factor: 5.157

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  Studies on the degradation of tyrosine aminotransferase in hepatoma cells in culture. Influence of the composition of the medium and adenosine triphosphate dependence.

Authors:  A Hershko; G M Tomkins
Journal:  J Biol Chem       Date:  1971-02-10       Impact factor: 5.157

8.  A heat-stable polypeptide component of an ATP-dependent proteolytic system from reticulocytes.

Authors:  A Ciehanover; Y Hod; A Hershko
Journal:  Biochem Biophys Res Commun       Date:  1978-04-28       Impact factor: 3.575

9.  Inactivation and proteolytic cleavage of phage lambda repressor in vitro in an ATP-dependent reaction.

Authors:  J W Roberts; C W Roberts; D W Mount
Journal:  Proc Natl Acad Sci U S A       Date:  1977-06       Impact factor: 11.205

10.  A soluble ATP-dependent proteolytic system responsible for the degradation of abnormal proteins in reticulocytes.

Authors:  J D Etlinger; A L Goldberg
Journal:  Proc Natl Acad Sci U S A       Date:  1977-01       Impact factor: 11.205
View more
  127 in total

1.  A novel active site-directed probe specific for deubiquitylating enzymes reveals proteasome association of USP14.

Authors:  A Borodovsky; B M Kessler; R Casagrande; H S Overkleeft; K D Wilkinson; H L Ploegh
Journal:  EMBO J       Date:  2001-09-17       Impact factor: 11.598

2.  A ubiquitin carrier protein from wheat germ is structurally and functionally similar to the yeast DNA repair enzyme encoded by RAD6.

Authors:  M L Sullivan; R D Vierstra
Journal:  Proc Natl Acad Sci U S A       Date:  1989-12       Impact factor: 11.205

3.  An ATP-stabilized inhibitor of the proteasome is a component of the 1500-kDa ubiquitin conjugate-degrading complex.

Authors:  J Driscoll; J Frydman; A L Goldberg
Journal:  Proc Natl Acad Sci U S A       Date:  1992-06-01       Impact factor: 11.205

4.  Accelerating scientific publication in biology.

Authors:  Ronald D Vale
Journal:  Proc Natl Acad Sci U S A       Date:  2015-10-27       Impact factor: 11.205

5.  Pharmacodynamic and efficacy studies of the novel proteasome inhibitor NPI-0052 (marizomib) in a human plasmacytoma xenograft murine model.

Authors:  Ajita V Singh; Michael A Palladino; George Kenneth Lloyd; Barbara C Potts; Dharminder Chauhan; Kenneth C Anderson
Journal:  Br J Haematol       Date:  2010-03-12       Impact factor: 6.998

6.  The discovery of ubiquitin-dependent proteolysis.

Authors:  Keith D Wilkinson
Journal:  Proc Natl Acad Sci U S A       Date:  2005-10-17       Impact factor: 11.205

Review 7.  Weighing in on ubiquitin: the expanding role of mass-spectrometry-based proteomics.

Authors:  Donald S Kirkpatrick; Carilee Denison; Steven P Gygi
Journal:  Nat Cell Biol       Date:  2005-08       Impact factor: 28.824

Review 8.  Ubiquitin signalling in the NF-kappaB pathway.

Authors:  Zhijian J Chen
Journal:  Nat Cell Biol       Date:  2005-08       Impact factor: 28.824

9.  Cloning and characterization of a 20-kDa ubiquitin carrier protein from wheat that catalyzes multiubiquitin chain formation in vitro.

Authors:  S Van Nocker; R D Vierstra
Journal:  Proc Natl Acad Sci U S A       Date:  1991-11-15       Impact factor: 11.205

Review 10.  The multiple layers of ubiquitin-dependent cell cycle control.

Authors:  Katherine Wickliffe; Adam Williamson; Lingyan Jin; Michael Rape
Journal:  Chem Rev       Date:  2009-04       Impact factor: 60.622
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.