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Literature DB >> 16056266

Weighing in on ubiquitin: the expanding role of mass-spectrometry-based proteomics.

Donald S Kirkpatrick¹, Carilee Denison, Steven P Gygi.

Abstract

Mass-spectrometry-based proteomics has become an essential tool for the qualitative and quantitative analysis of cellular systems. The biochemical complexity and functional diversity of the ubiquitin system are well suited to proteomic studies. This review summarizes advances involving the identification of ubiquitinated proteins, the elucidation of ubiquitin-modification sites and the determination of polyubiquitin chain linkages, as well as offering a perspective on the application of emerging technologies for mechanistic and functional studies of protein ubiquitination.

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Year: 2005 PMID： 16056266 PMCID： PMC1224607 DOI： 10.1038/ncb0805-750

Source DB: PubMed Journal: Nat Cell Biol ISSN： 1465-7392 Impact factor: 28.824

100 in total

Weighing in on ubiquitin: the expanding role of mass-spectrometry-based proteomics.

1. Accurate quantitation of protein expression and site-specific phosphorylation.

2. Global analysis of protein sumoylation in Saccharomyces cerevisiae.

3. Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.

Review 4. Proteomic analysis of cellular signaling.

5. Ubiquitin dependence of selective protein degradation demonstrated in the mammalian cell cycle mutant ts85.

6. Inhibition of proteolysis and cell cycle progression in a multiubiquitination-deficient yeast mutant.

7. Topors functions as an E3 ubiquitin ligase with specific E2 enzymes and ubiquitinates p53.

8. Proteolysis-independent regulation of the transcription factor Met4 by a single Lys 48-linked ubiquitin chain.

9. A proteomic strategy for gaining insights into protein sumoylation in yeast.

10. Activity-based ubiquitin-specific protease (USP) profiling of virus-infected and malignant human cells.

1. Analysis of ubiquitinated proteome by quantitative mass spectrometry.

2. Unraveling the ubiquitome of the human malaria parasite.

3. Polyubiquitin linkage profiles in three models of proteolytic stress suggest the etiology of Alzheimer disease.

Review 4. Modification site localization scoring: strategies and performance.

Review 5. The elimination of accumulated and aggregated proteins: a role for aggrephagy in neurodegeneration.

Review 6. Proteasome protease mediated regulation of cytokine induction and inflammation.

7. p62/SQSTM1 is required for Parkin-induced mitochondrial clustering but not mitophagy; VDAC1 is dispensable for both.

Review 8. Ubiquitin and ubiquitin-like modifications of the p53 family.

Review 9. Dissecting the ubiquitin pathway by mass spectrometry.

10. Mass spectrometric analysis of type 1 inositol 1,4,5-trisphosphate receptor ubiquitination.