Literature DB >> 6768582

Synthesis of maleimide derivative of cortisol for enzyme coupling is cortisol enzyme immunoassay.

N Monji, A Castro.   

Abstract

A new cortisol derivative, cortisol-21-m-maleimidobenzoate (CMB), was synthesized and conjugated with sulfhydryl groups of beta-galactosidase (BG). Both CMB-BG and CHS-BG conjugates have a high immunoreactivity to cortisol antibody, and although CHS-BG does not displace well with the added cortisol, CMB-BG does.

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Year:  1980        PMID： 6768582     DOI： 10.1007/bf01952319

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  9 in total

1.  Enzyme-linked immunosorbent choriomammotropin assay.

Authors:  D Dittmar; N Monji; A Cid; H Malkus; A Castro
Journal:  Clin Chem       Date:  1979-02       Impact factor: 8.327

2.  Enzyme coupled immunoassay of insulin using a novel coupling reagent.

Authors:  T Kitagawa; T Aikawa
Journal:  J Biochem       Date:  1976-01       Impact factor: 3.387

3.  An immuno-enzymatic assay of cortisol using E. coli beta-galactosidase as label.

Authors:  S Comoglio; F Celada
Journal:  J Immunol Methods       Date:  1976-03       Impact factor: 2.303

4.  Enzyme-linked immunoassay. Conjugation of rabbit anti-(human immunoglobulin G) antibody with beta-D-galactosidase from Escherichia coli and its use for human immunoglobulin G assay.

Authors:  K Kato; Y Hamaguchi; H Fukui; E Ishikawa
Journal:  Eur J Biochem       Date:  1976-02-16

5.  A radioimmunoassay for cortisol in plasma and urine.

Authors:  H J Ruder; R L Guy; M B Lipsett
Journal:  J Clin Endocrinol Metab       Date:  1972-08       Impact factor: 5.958

6.  Determination of sulfhydryl groups with 2,2'- or 4,4'-dithiodipyridine.

Authors:  D R Grassetti; J F Murray
Journal:  Arch Biochem Biophys       Date:  1967-03       Impact factor: 4.013

7.  Enzyme-linked immunoassay: conjugation of the Fab' fragment of rabbit IgG with beta-D-galactosidase from E. coli and its use for immunoassay.

Authors:  K Kato; H Fukui; Y Hamaguchi; E Ishikawa
Journal:  J Immunol       Date:  1976-06       Impact factor: 5.422

8.  A novel method in enzyme immunoassay: maleimide derivative of hapten for enzyme coupling.

Authors:  A Castro; N Monji
Journal:  Experientia       Date:  1979-04-15

9.  Enzyme immunoassay of progesterone at the picogram level using beta-galactosidase as label.

Authors:  F Dray; J M Andrieu; F Renaud
Journal:  Biochim Biophys Acta       Date:  1975-09-22
  9 in total
  1 in total

1.  Quantification of digoxin by enzyme immunoassay: synthesis of a maleimide derivative of digoxigenin succinate for enzyme coupling.

Authors:  N Monji; H Ali; A Castro
Journal:  Experientia       Date:  1980-10-15
  1 in total

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