Literature DB >> 108135

A novel method in enzyme immunoassay: maleimide derivative of hapten for enzyme coupling.

A Castro, N Monji.   

Abstract

Meta-maleimidobenzoyl derivative of L-thyroxine methyl ester (MBTM) was synthesized and coupled to beta-galactosidase at molar ratio of over 5 to 1. More than 97% of the enzyme was found to be labeled with MBTM. A thyroxine enzyme immunoassay was carried out with sensitivity in 0-10 microgram/100 ml range.

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Year:  1979        PMID： 108135     DOI： 10.1007/bf01922777

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  7 in total

1.  Enzyme coupled immunoassay of insulin using a novel coupling reagent.

Authors:  T Kitagawa; T Aikawa
Journal:  J Biochem       Date:  1976-01       Impact factor: 3.387

2.  An immuno-enzymatic assay of cortisol using E. coli beta-galactosidase as label.

Authors:  S Comoglio; F Celada
Journal:  J Immunol Methods       Date:  1976-03       Impact factor: 2.303

3.  Enzyme-linked immunoassay. Conjugation of rabbit anti-(human immunoglobulin G) antibody with beta-D-galactosidase from Escherichia coli and its use for human immunoglobulin G assay.

Authors:  K Kato; Y Hamaguchi; H Fukui; E Ishikawa
Journal:  Eur J Biochem       Date:  1976-02-16

4.  Radioimmunoassay for triiodothyronine (T 3 ): I. Affinity and specificity of the antibody for T 3 .

Authors:  H Gharib; R J Ryan; W E Mayberry; T Hockert
Journal:  J Clin Endocrinol Metab       Date:  1971-09       Impact factor: 5.958

5.  Determination of sulfhydryl groups with 2,2'- or 4,4'-dithiodipyridine.

Authors:  D R Grassetti; J F Murray
Journal:  Arch Biochem Biophys       Date:  1967-03       Impact factor: 4.013

6.  Enzyme-linked immunoassay: conjugation of the Fab' fragment of rabbit IgG with beta-D-galactosidase from E. coli and its use for immunoassay.

Authors:  K Kato; H Fukui; Y Hamaguchi; E Ishikawa
Journal:  J Immunol       Date:  1976-06       Impact factor: 5.422

7.  Enzyme immunoassay of progesterone at the picogram level using beta-galactosidase as label.

Authors:  F Dray; J M Andrieu; F Renaud
Journal:  Biochim Biophys Acta       Date:  1975-09-22
  7 in total
  1 in total

1.  Synthesis of maleimide derivative of cortisol for enzyme coupling is cortisol enzyme immunoassay.

Authors:  N Monji; A Castro
Journal:  Experientia       Date:  1980-03-15
  1 in total

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