Literature DB >> 6765239

The site of interaction of aminoacyl-tRNA with elongation factor Tu.

F P Wikman, G E Siboska, H U Petersen, B F Clark.   

Abstract

We have used RNases T1, T2 and A to digest two aminoacyl-tRNAs, Escherichia coli Phe-tRNAPhe and E. coli Met- tRNAMetm both in the naked forms and in ternary complexes with E. coli elongation factor Tu (EF-Tu) and GTP. An analysis of the 'footprinting' results has led to an interpretation that has localized the part of the three-dimensional structure of aminoacyl-tRNA covered by the protein in the ternary complex. In terms of the three-dimensional structure of tRNA established for yeast tRNAPhe, EF-Tu covers the aa-end, aa-stem, T-stem, and extra loop on the side of the L-shaped tRNA that exposes the extra loop.

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Year:  1982        PMID: 6765239      PMCID: PMC553168          DOI: 10.1002/j.1460-2075.1982.tb01302.x

Source DB:  PubMed          Journal:  EMBO J        ISSN: 0261-4189            Impact factor:   11.598


  16 in total

1.  Isolation, characterization and structural implications of a nuclease-digested complex of aminoacyl transfer RNA and Escherichia coli elongation factor Tu.

Authors:  E Jekowsky; P R Schimmel; D L Miller
Journal:  J Mol Biol       Date:  1977-08-15       Impact factor: 5.469

2.  Primary structure of a methionine transfer RNA from Escherichia coli.

Authors:  S Cory; K A Marcker; S K Dube; B F Clark
Journal:  Nature       Date:  1968-12-07       Impact factor: 49.962

3.  Evidence for a guanine nucleotide-aminoacyl-RNA complex as an intermediate in the enzymatic transfer of aminoacyl-RNA to ribosomes.

Authors:  J M Ravel; R L Shorey; W Shive
Journal:  Biochem Biophys Res Commun       Date:  1967-10-11       Impact factor: 3.575

4.  Compilation of tRNA sequences.

Authors:  M Sprinzl; D H Gauss
Journal:  Nucleic Acids Res       Date:  1982-01-22       Impact factor: 16.971

5.  A study of the interaction of Escherichia coli elongation factor-Tu with aminoacyl-tRNAs by partial digestion with cobra venom ribonuclease.

Authors:  A S Boutorin; B F Clark; J P Ebel; T A Kruse; H U Petersen; P Remy; S Vassilenko
Journal:  J Mol Biol       Date:  1981-11-05       Impact factor: 5.469

6.  The structure of the CCA end of tRNA, aminoacyl-tRNA and aminoacyl-tRNA in the ternary complex.

Authors:  T A Kruse; B F Clark; B Appel; V A Erdmann
Journal:  FEBS Lett       Date:  1980-08-11       Impact factor: 4.124

7.  Interaction of initiator Met-tRNArMet (Escherichia coli) and Gly-tRNAIGly (Staphylococcus epidermidis) with bacterial elongation factor Tu:GTP complex.

Authors:  S Tanada; M Kawakami; T Yoneda; S Takemura
Journal:  J Biochem       Date:  1981-05       Impact factor: 3.387

8.  The effect of specific structural modification on the biological activity of E. coli arginine tRNA.

Authors:  T A Kruse; B F Clark
Journal:  Nucleic Acids Res       Date:  1978-03       Impact factor: 16.971

9.  Altered mobility of polydeoxyribonucleotides in high resolution polyacrylamide gels due to removal of terminal phosphates.

Authors:  D P Tapper; D A Clayton
Journal:  Nucleic Acids Res       Date:  1981-12-21       Impact factor: 16.971

10.  Ternary complex formation between elongation factor Tu, GTP and aminoacyl-tRNA: an equilibrium study.

Authors:  A Pingoud; C Urbanke; G Krauss; F Peters; G Maass
Journal:  Eur J Biochem       Date:  1977-09
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  12 in total

1.  Glycine tRNA mutants with normal anticodon loop size cause -1 frameshifting.

Authors:  D J O'Mahony; B H Mims; S Thompson; E J Murgola; J F Atkins
Journal:  Proc Natl Acad Sci U S A       Date:  1989-10       Impact factor: 11.205

2.  How are tRNAs and mRNA arranged in the ribosome? An attempt to correlate the stereochemistry of the tRNA-mRNA interaction with constraints imposed by the ribosomal topography.

Authors:  V Lim; C Venclovas; A Spirin; R Brimacombe; P Mitchell; F Müller
Journal:  Nucleic Acids Res       Date:  1992-06-11       Impact factor: 16.971

3.  Crosslinking of tRNA containing a long extra arm to elongation factor Tu by trans-diamminedichloroplatinum(II).

Authors:  N J Rasmussen; F P Wikman; B F Clark
Journal:  Nucleic Acids Res       Date:  1990-08-25       Impact factor: 16.971

4.  Ser-tRNAs from bovine mitochondrion form ternary complexes with bacterial elongation factor Tu and GTP.

Authors:  E Gebhardt-Singh; M Sprinzl
Journal:  Nucleic Acids Res       Date:  1986-09-25       Impact factor: 16.971

5.  Anticodon-anticodon interaction induces conformational changes in tRNA: yeast tRNAAsp, a model for tRNA-mRNA recognition.

Authors:  D Moras; A C Dock; P Dumas; E Westhof; P Romby; J P Ebel; R Giegé
Journal:  Proc Natl Acad Sci U S A       Date:  1986-02       Impact factor: 11.205

6.  Improvement of reading frame maintenance is a common function for several tRNA modifications.

Authors:  J Urbonavicius; Q Qian; J M Durand; T G Hagervall; G R Björk
Journal:  EMBO J       Date:  2001-09-03       Impact factor: 11.598

7.  Aminoacyl RNA domain of turnip yellow mosaic virus Val-RNA interacting with elongation factor Tu.

Authors:  R L Joshi; H Faulhammer; F Chapeville; M Sprinzl; A L Haenni
Journal:  Nucleic Acids Res       Date:  1984-10-11       Impact factor: 16.971

8.  A novel cloverleaf structure found in mammalian mitochondrial tRNA(Ser) (UCN).

Authors:  T Yokogawa; Y Watanabe; Y Kumazawa; T Ueda; I Hirao; K Miura; K Watanabe
Journal:  Nucleic Acids Res       Date:  1991-11-25       Impact factor: 16.971

9.  Crosslinking of elongation factor Tu to tRNA(Phe) by trans-diamminedichloroplatinum (II). Characterization of two crosslinking sites in the tRNA.

Authors:  F P Wikman; P Romby; M H Metz; J Reinbolt; B F Clark; J P Ebel; C Ehresmann; B Ehresmann
Journal:  Nucleic Acids Res       Date:  1987-07-24       Impact factor: 16.971

10.  Antideterminants present in minihelix(Sec) hinder its recognition by prokaryotic elongation factor Tu.

Authors:  J Rudinger; R Hillenbrandt; M Sprinzl; R Giegé
Journal:  EMBO J       Date:  1996-02-01       Impact factor: 11.598

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