Literature DB >> 3302946

Crosslinking of elongation factor Tu to tRNA(Phe) by trans-diamminedichloroplatinum (II). Characterization of two crosslinking sites in the tRNA.

F P Wikman, P Romby, M H Metz, J Reinbolt, B F Clark, J P Ebel, C Ehresmann, B Ehresmann.   

Abstract

Trans-diamminedichloroplatinum (II) was used to induce reversible crosslinks between EF-Tu and Phe-tRNA(Phe) within the ternary EF-Tu/GTP/Phe-tRNA(Phe) complex. Up to 40% of the complex was specifically converted into crosslinked species. Two crosslinking sites have been unambiguously identified. The major one encompassing nucleotides 58 to 65 is located in the 3'-part of the T-stem, and the minor one encompassing nucleotides 31 to 42 includes the anticodon loop and part of the 3'-strand of the anticodon stem.

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Year:  1987        PMID： 3302946      PMCID： PMC306023          DOI： 10.1093/nar/15.14.5787

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  27 in total

1.  The use of nuclease P1 in sequence analysis of end group labeled RNA.

Authors:  M Silberklang; A M Gillum; U L RajBhandary
Journal:  Nucleic Acids Res       Date:  1977-12       Impact factor: 16.971

2.  Low resolution structure of partially trypsin-degraded polypeptide elongation factor, EF-TU, from Escherichia coli.

Authors:  W Kabsch; W H Gast; G E Schulz; R Leberman
Journal:  J Mol Biol       Date:  1977-12-25       Impact factor: 5.469

3.  Comparison of Escherichia coli tRNAPhe in the free state, in the ternary complex and in the ribosomal A and P sites by chemical probing.

Authors:  S Douthwaite; R A Garrett; R Wagner
Journal:  Eur J Biochem       Date:  1983-03-15

4.  The influence of elongation-factor-Tu . GTP and anticodon-anticodon interactions on the anticodon loop conformation of yeast tRNATyr.

Authors:  I Weygand-Durasevic; T A Kruse; B F Clark
Journal:  Eur J Biochem       Date:  1981-05

5.  A study of the interaction of Escherichia coli elongation factor-Tu with aminoacyl-tRNAs by partial digestion with cobra venom ribonuclease.

Authors:  A S Boutorin; B F Clark; J P Ebel; T A Kruse; H U Petersen; P Remy; S Vassilenko
Journal:  J Mol Biol       Date:  1981-11-05       Impact factor: 5.469

6.  A small-angle X-ray scattering study of the complex formation between elongation factor Tu . GTP and valyl-tRNA Val I from Escherichia coli.

Authors:  R Osterberg; B Sjöberg; R Ligaarden; P Elias
Journal:  Eur J Biochem       Date:  1981-06

7.  The yeast aminoacyl-tRNA synthetases. Methodology for their complete or partial purification and comparison of their relative activities under various extraction conditions.

Authors:  D Kern; A Dietrich; F Fasiolo; M Renaud; R Giegé; J P Ebel
Journal:  Biochimie       Date:  1977       Impact factor: 4.079

8.  Yeast phenylalanine transfer RNA: atomic coordinates and torsion angles.

Authors:  G J Quigley; N C Seeman; A H Wang; F L Suddath; A Rich
Journal:  Nucleic Acids Res       Date:  1975-12       Impact factor: 16.971

9.  Ternary complex formation between elongation factor Tu, GTP and aminoacyl-tRNA: an equilibrium study.

Authors:  A Pingoud; C Urbanke; G Krauss; F Peters; G Maass
Journal:  Eur J Biochem       Date:  1977-09

10.  Formation and properties of a covalent complex between elongation factor Tu and Phe-tRNA bearing a photoaffinity probe on its 3-(3-amino-3-carboxypropyl)uridine residue.

Authors:  T Kao; D L Miller; M Abo; J Ofengand
Journal:  J Mol Biol       Date:  1983-05-25       Impact factor: 5.469
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  5 in total

1.  Crosslinking of tRNA containing a long extra arm to elongation factor Tu by trans-diamminedichloroplatinum(II).

Authors:  N J Rasmussen; F P Wikman; B F Clark
Journal:  Nucleic Acids Res       Date:  1990-08-25       Impact factor: 16.971

2.  Crosslinking of transcription factor TFIIIA to ribosomal 5S RNA from X. laevis by trans-diamminedichloroplatinum (II).

Authors:  F Baudin; P Romby; P J Romaniuk; B Ehresmann; C Ehresmann
Journal:  Nucleic Acids Res       Date:  1989-12-11       Impact factor: 16.971

3.  Antideterminants present in minihelix(Sec) hinder its recognition by prokaryotic elongation factor Tu.

Authors:  J Rudinger; R Hillenbrandt; M Sprinzl; R Giegé
Journal:  EMBO J       Date:  1996-02-01       Impact factor: 11.598

4.  In vitro analysis of mutations causing myoclonus epilepsy with ragged-red fibers in the mitochondrial tRNA(Lys)gene: two genotypes produce similar phenotypes.

Authors:  J P Masucci; M Davidson; Y Koga; E A Schon; M P King
Journal:  Mol Cell Biol       Date:  1995-05       Impact factor: 4.272

5.  Many of the conserved nucleotides of tRNA(Phe) are not essential for ternary complex formation and peptide elongation.

Authors:  I A Nazarenko; K M Harrington; O C Uhlenbeck
Journal:  EMBO J       Date:  1994-05-15       Impact factor: 11.598
  5 in total

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