Literature DB >> 6736559

An apparatus for counter-current distribution in a centrifugal acceleration field.

H E Akerlund.   

Abstract

An apparatus is described that reduces the time required for counter-current distribution in aqueous polymer two-phase systems by a factor of more than five. Phase settling, which has been the time consuming step, is here facilitated by centrifugation. The crucial point in the construction, that allows automatization, is that the transfer step in the counter-current distribution cycle is performed during centrifugation.

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Year:  1984        PMID: 6736559     DOI: 10.1016/0165-022x(84)90004-6

Source DB:  PubMed          Journal:  J Biochem Biophys Methods        ISSN: 0165-022X


  6 in total

1.  The contribution of photosynthetic pigments to the development of biochemical separation methods: 1900-1980.

Authors:  Per-Ake Albertsson
Journal:  Photosynth Res       Date:  2003       Impact factor: 3.573

2.  Lateral heterogeneity of rat liver plasma membranes analysed by counter-current distribution.

Authors:  P Gierow; B Jergil; P A Albertsson
Journal:  Biochem J       Date:  1988-01-15       Impact factor: 3.857

3.  Partition of macromolecules and cell particles in aqueous two-phase systems based on hydroxypropyl starch and poly(ethylene glycol).

Authors:  S Sturesson; F Tjerneld; G Johansson
Journal:  Appl Biochem Biotechnol       Date:  1990-12       Impact factor: 2.926

4.  Characterization of a non-detergent PS II-cytochrome b/f preparation (BS).

Authors:  S G Yu; G Björn; P Å Albertsson
Journal:  Photosynth Res       Date:  1993-09       Impact factor: 3.573

5.  Purification and characterization of pea chloroplastic phosphoriboisomerase.

Authors:  C L Skrukrud; I M Gordon; S Dorwin; X H Yuan; G Johansson; L E Anderson
Journal:  Plant Physiol       Date:  1991-10       Impact factor: 8.340

6.  Heterogeneity of a crude synaptosomal preparation, studied by affinity partitioning using hexaethonium-poly(ethylene glycol).

Authors:  B Olde; G Johansson
Journal:  Mol Cell Biochem       Date:  1989-06-01       Impact factor: 3.396

  6 in total

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