Literature DB >> 2091530

Partition of macromolecules and cell particles in aqueous two-phase systems based on hydroxypropyl starch and poly(ethylene glycol).

S Sturesson1, F Tjerneld, G Johansson.   

Abstract

The partition behavior of proteins, nucleic acids, cell membranes, cell organelles and whole cells has been studied in liquid-liquid two-phase systems composed of water, poly-(ethylene glycol), and an hydroxypropyl starch. The properties of the systems are in many respects comparable with the traditional poly(ethylene glycol)-dextran systems, but the cost is reduced to around one-fifth.

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Year:  1990        PMID： 2091530     DOI： 10.1007/BF02921507

Source DB:  PubMed          Journal:  Appl Biochem Biotechnol        ISSN： 0273-2289            Impact factor:   2.926


  14 in total

1.  Separation of subchloroplast membrane particles by counter-current distribution.

Authors:  B Andersson; H E Akerlund; P A Albertsson
Journal:  Biochim Biophys Acta       Date:  1976-01-15

2.  PURIFICATION, COMPOSITION, AND MOLECULAR WEIGHT OF THE BETA-GALACTOSIDASE OF ESCHERICHIA COLI K12.

Authors:  G R CRAVEN; E STEERS; C B ANFINSEN
Journal:  J Biol Chem       Date:  1965-06       Impact factor: 5.157

3.  Glucose 6-phosphate dehydrogenase (Zwischenferment). I. Isolation of the crystalline enzyme from yeast.

Authors:  E A NOLTMANN; C J GUBLER; S A KUBY
Journal:  J Biol Chem       Date:  1961-05       Impact factor: 5.157

4.  Affinity partitioning with polymer-bound Cibacron blue F3G-A for rapid, large-scale purification of phosphofructokinase from Baker's yeast.

Authors:  G Kopperschläger; G Johansson
Journal:  Anal Biochem       Date:  1982-07-15       Impact factor: 3.365

5.  Study on the partition of beta-galactosidase from Escherichia coli in aqueous two-phase systems and elaboration of a simple procedure for the purification of the enzyme to homogeneity.

Authors:  B Schwenzer; G Kopperschläger
Journal:  Biomed Biochim Acta       Date:  1989

6.  Affinity liquid-liquid extraction of lactate dehydrogenase on a large scale.

Authors:  F Tjerneld; G Johansson; M Joelsson
Journal:  Biotechnol Bioeng       Date:  1987-11       Impact factor: 4.530

7.  Evaluation of crude dextran as phase-forming polymer for the extraction of enzymes in aqueous two-phase systems in large scale.

Authors:  K H Kroner; H Hustedt; M R Kula
Journal:  Biotechnol Bioeng       Date:  1982-05       Impact factor: 4.530

8.  Affinity partitioning and centrifugal counter-current distribution of membrane-bound opiate receptors using naloxone-poly(ethylene glycol).

Authors:  B Olde; G Johansson
Journal:  Neuroscience       Date:  1985-08       Impact factor: 3.590

9.  Fractionation of rat liver plasma-membrane regions by two-phase partitioning.

Authors:  P Gierow; M Sommarin; C Larsson; B Jergil
Journal:  Biochem J       Date:  1986-05-01       Impact factor: 3.857

10.  A Process for large-scale isolation of beta-galactosidase from E. coli in an aqueous two-phase system.

Authors:  A Veide; A L Smeds; S O Enfors
Journal:  Biotechnol Bioeng       Date:  1983-07       Impact factor: 4.530
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  3 in total

Review 1.  A new look at xylanases: an overview of purification strategies.

Authors:  Paula Sá-Pereira; Helena Paveia; Maria Costa-Ferreira; Maria Aires-Barros
Journal:  Mol Biotechnol       Date:  2003-07       Impact factor: 2.695

2.  Evaluation of the effect of ionic liquids as adjuvants in polymer-based aqueous biphasic systems using biomolecules as molecular probes.

Authors:  Rita de Cássia S Sousa; Matheus M Pereira; Mara G Freire; João A P Coutinho
Journal:  Sep Purif Technol       Date:  2017-07-14       Impact factor: 7.312

3.  Optimization of serine protease purification from mango (Mangifera indica cv. Chokanan) peel in polyethylene glycol/dextran aqueous two phase system.

Authors:  Amid Mehrnoush; Shuhaimi Mustafa; Md Zaidul Islam Sarker; Abdul Manap Mohd Yazid
Journal:  Int J Mol Sci       Date:  2012-03-19       Impact factor: 6.208
  3 in total

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