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Literature DB >> 2770719

Heterogeneity of a crude synaptosomal preparation, studied by affinity partitioning using hexaethonium-poly(ethylene glycol).

Abstract

The heterogeneity of a synaptosomal preparation was studied by the use of affinity partitioning in combination with centrifugal counter-current distribution. Hexaethonium-poly(ethyleneglycol) was used as the extracting agent. The fractions were analyzed for: light scattering, protein, choline acetyltransferase, L-glutamate decarboxylase, glutamine synthetase, 2',3'-cyclicnucleotide-3'-phosphohydrolase, acetylcholinesterase and succinate dehydrogenase. The material was fractionated into three main fractions which differed in their content of marker-enzymes.

Entities: Chemical Gene

Mesh：

Substances：

Year: 1989 PMID： 2770719 DOI： 10.1007/BF00219258

Source DB: PubMed Journal: Mol Cell Biochem ISSN： 0300-8177 Impact factor: 3.396

40 in total

1. The isolation of nerve endings from brain: an electron-microscopic study of cell fragments derived by homogenization and centrifugation.

Authors: E G GRAY; V P WHITTAKER
Journal: J Anat Date: 1962-01 Impact factor: 2.610

2. Affinity partitioning of acetylcholine receptor enriched membranes and their purification.

Authors: S D Flanagan; P Taylor; S H Barondes
Journal: Nature Date: 1975-04-03 Impact factor: 49.962

3. Glial contamination of synaptosomal fractions.

Authors: F A Henn; D J Anderson; D G Rustad
Journal: Brain Res Date: 1976-01-16 Impact factor: 3.252

4. Inside-out membrane vesicles isolated from spinach thylakoids.

Authors: B Andersson; H E Akerlund
Journal: Biochim Biophys Acta Date: 1978-09-07

Review 5. Microanalytical methods for neuronal analysis.

Authors: J Y Wu
Journal: Physiol Rev Date: 1978-10 Impact factor: 37.312

6. Optimization of freezing, storage, and thawing conditions for the preparation of metabolically active synaptosomes from frozen rat and human brain.

Authors: P R Dodd; J A Hardy; E B Baig; A M Kidd; E D Bird; W E Watson; G A Johnston
Journal: Neurochem Pathol Date: 1986-06

Heterogeneity of a crude synaptosomal preparation, studied by affinity partitioning using hexaethonium-poly(ethylene glycol).

1. The isolation of nerve endings from brain: an electron-microscopic study of cell fragments derived by homogenization and centrifugation.

2. Affinity partitioning of acetylcholine receptor enriched membranes and their purification.

3. Glial contamination of synaptosomal fractions.

4. Inside-out membrane vesicles isolated from spinach thylakoids.

Review 5. Microanalytical methods for neuronal analysis.

6. Optimization of freezing, storage, and thawing conditions for the preparation of metabolically active synaptosomes from frozen rat and human brain.

7. Cellular localization and regulation of glutamine synthetase in primary cultures of brain cells from newborn mice.

8. Metabolically active synaptosomes can be prepared from frozen rat and human brain.

9. The preparation of highly purified GABAergic and cholinergic synaptosomes from mammalian brain.

10. Highly purified mitochondria from rat brain prepared by phase partition.

1. Astrocytic and Oligodendrocytic P2X7 Receptors Determine Neuronal Functions in the CNS.

Review 2. Involvement of P2X7 receptors in chronic pain disorders.