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Literature DB >> 6726892

Plaque assay for black beetle virus.

Abstract

A rapidly growing strain of virus was used to develop a reliable plaque assay for Black beetle virus on monolayers of cultured Drosophila cells. Cell density of the monolayer was critical for successful plaque formation. The dose-response curve for plaque formation was linear, supporting earlier proposals that both RNA segments of the split genome reside in the same particle. The method greatly facilitates isolation of reassortant and variant strains of virus.

Entities: Species

Mesh：

Substances：
RNA, Viral

Year: 1984 PMID： 6726892 PMCID： PMC254426 DOI： 10.1128/JVI.51.1.251-253.1984

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

14 in total

1. A small RNA virus with a divided genome from Heteronychus arator (F.) [Coleoperai Scarabaeidae].

Authors: J F Longworth; G P Carey
Journal: J Gen Virol Date: 1976-10 Impact factor: 3.891

2. Cell cycle-dependent activation of rous sarcoma virus-infected stationary chicken cells: avian leukosis virus group-specific antigens and ribonucleic acid.

Authors: E H Humphries; H M Temin
Journal: J Virol Date: 1972-07 Impact factor: 5.103

3. Replication of the parvovirus MVM. I. Dependence of virus multiplication and plaque formation on cell growth.

Authors: P Tattersall
Journal: J Virol Date: 1972-10 Impact factor: 5.103

4. Further physicochemical characterization of Nodamura virus. Evidence that the divided genome occurs in a single component.

Authors: J F Newman; F Brown
Journal: J Gen Virol Date: 1978-01 Impact factor: 3.891

5. In vitro translation of the Two RNAs of Nodamura virus, a novel mammalian virus with a divided genome.

Authors: J F Newman; T Matthews; D R Omilianowski; T Salerno; P Kaesberg; R Rueckert
Journal: J Virol Date: 1978-01 Impact factor: 5.103

6. Cell lines derived from late embryonic stages of Drosophila melanogaster.

Authors: I Schneider
Journal: J Embryol Exp Morphol Date: 1972-04

7. Synthesis of Black Beetle Virus Proteins in Cultured Drosophila Cells: Differential Expression of RNAs 1 and 2.

Authors: P D Friesen; R R Rueckert
Journal: J Virol Date: 1981-03 Impact factor: 5.103

8. Black beetle virus: propagation in Drosophila line 1 cells and an infection-resistant subline carrying endogenous black beetle virus-related particles.

Authors: P Friesen; P Scotti; J Longworth; R Rueckert
Journal: J Virol Date: 1980-09 Impact factor: 5.103

9. Flock House virus: a nodavirus isolated from Costelytra zealandica (White) (Coleoptera: Scarabaeidae).

Authors: P D Scotti; S Dearing; D W Mossop
Journal: Arch Virol Date: 1983 Impact factor: 2.574

32 in total

1. DNA-Directed expression of functional flock house virus RNA1 derivatives in Saccharomyces cerevisiae, heterologous gene expression, and selective effects on subgenomic mRNA synthesis.

Authors: B D Price; M Roeder; P Ahlquist
Journal: J Virol Date: 2000-12 Impact factor: 5.103

10. Flock house virus RNA polymerase is a transmembrane protein with amino-terminal sequences sufficient for mitochondrial localization and membrane insertion.

Authors: David J Miller; Paul Ahlquist
Journal: J Virol Date: 2002-10 Impact factor: 5.103

Plaque assay for black beetle virus.

1. A small RNA virus with a divided genome from Heteronychus arator (F.) [Coleoperai Scarabaeidae].

2. Cell cycle-dependent activation of rous sarcoma virus-infected stationary chicken cells: avian leukosis virus group-specific antigens and ribonucleic acid.

3. Replication of the parvovirus MVM. I. Dependence of virus multiplication and plaque formation on cell growth.

4. Further physicochemical characterization of Nodamura virus. Evidence that the divided genome occurs in a single component.

5. In vitro translation of the Two RNAs of Nodamura virus, a novel mammalian virus with a divided genome.

6. Cell lines derived from late embryonic stages of Drosophila melanogaster.

7. Synthesis of Black Beetle Virus Proteins in Cultured Drosophila Cells: Differential Expression of RNAs 1 and 2.

8. Black beetle virus: propagation in Drosophila line 1 cells and an infection-resistant subline carrying endogenous black beetle virus-related particles.

9. Flock House virus: a nodavirus isolated from Costelytra zealandica (White) (Coleoptera: Scarabaeidae).

1. DNA-Directed expression of functional flock house virus RNA1 derivatives in Saccharomyces cerevisiae, heterologous gene expression, and selective effects on subgenomic mRNA synthesis.

2. Maturation cleavage required for infectivity of a nodavirus.

3. Evidence that the packaging signal for nodaviral RNA2 is a bulged stem-loop.

4. Nonhomologous RNA recombination during negative-strand synthesis of flock house virus RNA.

5. Cotranslational disassembly of flock house virus in a cell-free system.

6. Flock house virus induces apoptosis by depletion of Drosophila inhibitor-of-apoptosis protein DIAP1.

7. cis-acting requirements for the replication of flock house virus RNA 2.

8. Cellular expression of a functional nodavirus RNA replicon from vaccinia virus vectors.

9. Replication of nodamura virus after transfection of viral RNA into mammalian cells in culture.

10. Flock house virus RNA polymerase is a transmembrane protein with amino-terminal sequences sufficient for mitochondrial localization and membrane insertion.